参芪合剂对环磷酰胺所致免疫抑制小鼠免疫和抗氧化功能的影响  

Effects of mixture from ginsebosides and astragalus polysaccharides on immune and antioxidative function in immunosuppressive mice induced with CTX

作　　者：张海玉[1] 朴惠顺[2] 金在久[2] 张善玉[2] 

机构地区：[1]吉林大学第一医院儿外科,吉林长春130021 [2]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133000

出　　处：《吉林大学学报（医学版）》2009年第5期857-861,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200805136)

摘　　要：目的:观察参芪合剂(MGA)对环磷酰胺(CTX)所致免疫抑制小鼠免疫系统和抗氧化功能的影响,为MGA的临床应用提供实验依据。方法:60只昆明种小鼠随机分为5组:正常对照组、模型组和100、200、400 mg.kg-1MGA 3个剂量组,MGA组分别腹腔注射给药15 d,正常对照组和模型组腹腔注射等量生理盐水,第3和10天除正常对照组外的4个组均腹腔注射CTX 100 mg.kg-1,正常对照组腹腔注射等量生理盐水,测定小鼠免疫器官重量、网状内皮系统吞噬功能、外周白细胞(WBC)、全血谷胱甘肽过氧化物酶(GSH-Px)及血浆超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与正常对照组比较,免疫抑制模型组小鼠的胸腺、脾脏重量、吞噬指数及吞噬活性均明显降低(P<0.05或P<0.01),外周WBC计数明显减少(P<0.001),全血GSH-Px及血浆SOD活性明显降低(P<0.01),血浆MDA含量明显提高(P<0.05),提示CTX可造成小鼠免疫抑制及抗氧化功能降低;与模型组比较,MGA 200、400 mg.kg-1组小鼠脾脏重量、吞噬指数、吞噬活性及外周WBC计数均升高(P<0.05或P<0.01),全血GSH-Px活性明显升高(P<0.05),血浆MDA含量明显降低(P<0.05);400 mg.kg-1组小鼠胸腺重量和血浆SOD活性明显增加(P<0.05或P<0.01)。结论:MGA具有改善免疫抑制小鼠免疫和抗氧化功能的作用,提示该合剂可用作抗肿瘤补助治疗药物。Objective To observe the effects of mixture from ginsebosides and astragalus polysaccharides（MGA） on immune and antioxidative function in immunosuppressive mice induced with cyclophosphamide（CTX）,and provide the experimental basis for clinical application of MGA.Methods Sixty Kunming mice were randomly divided into normal control group,model group and 100,200,400 mg·kg^-1 MGA groups.The mice in MGA groups were administered by intraperitoneal injection for 15 d,The mice in normal control group and model group were administered with same volume physiological saline.On the 3rd and 10 th day,the mice in all groups except control group were intraperitoneally injected with CTX 100 mg·kg^-1,same volume physiological saline in control group.On the 15th day,the weights of immune organs,phagocytic funtion of macrophage system,periphery leukocytes,the activitie of GSH-Px in whole blood and SOD in plasma,and the content of MDA in plasma were detected.Results As compared with the controls,the weights of thymus and spleen,phagocytic index and phagocytic activity in immunosuppressive mice were significantly decreased（P〈0.05 or P〈0.01）;the peripheral blood WBC count was decreased significantly（P〈0.001）.The activities of blood GSH-Px and plasma SOD were significantly decreased（P〈0.01）,the plasma MDA content was markedly increased（P〈0.05）.It was indicated that CTX could cause immune suppression in mice and decrease anti-oxidation function.While compared with model group,the immune organ weights,phagocytic index,phagocytic activity and peripheral blood WBC count in 200 mg·kg^-1 and 400 mg·kg^-1 MGA groups were increased（P〈0.05 or P〈0.01）,the activity of blood GSH-Px was significantly increased（P〈0.05）,the plasma MDA content was markedly decreased（P〈0.05）.The weight of thymus and the plasma SOD in 400 mg·kg^-1 MGA group were significantly increased（P〈0.05 or P〈0.01）.Conclusion MGA has the effect of improving immune and antioxidative function in immunosuppressive mice

关 键 词：参芪合剂 环磷酰胺 抗氧化功能 免疫 

分 类 号：R95[医药卫生—药学]