HPV16／18、PCNA、P^53、ER在宫颈病变中表达的临床病理意义  

作　　者：应香岚 杨幼萍 瞿海江 林军 应亚君 应敏建 

机构地区：[1]浙江省温岭市第二人民医院病理科,317502

出　　处：《浙江临床医学》2009年第10期1015-1018,共4页Zhejiang Clinical Medical Journal

摘　　要：目的 探讨HPV16／18、PCNA、P^53、ER在不同程度宫颈病变组织中的表达。方法将242例患有宫颈鳞癌、宫颈上皮内病变及慢性宫颈炎的组织，用原位杂交方法检测HPV16／18及采用免疫组织化学Supervision二步法检测其PCNA、P^53、ER蛋白的表达。结果HPV16／18、PCNA、P^53及ER在宫颈鳞癌组中的阳性表达率分别为96．77％、91．94％、90．32％、6．45％，以HPV16／18的表达阳性率最高，PCNA表达强阳性；在高级别上皮内瘤变中的阳性表达率分别为96．77％、100％、80．65％，41．94％，以PCNA的表达阳性率最高，并呈强阳性；在低级别上皮内瘤变中的阳性表达率分别为62．07％、100％、62．07％79．31％，以PCNA表达阳性率最高，并呈中等强度；在对照组慢性宫颈炎中只有ER表达为100％，HPV16／18表达只有3．33％，而PCNA和P^53表达均呈弱阳性及阴性。宫颈鳞癌、高级别上皮内瘤变、低级别上皮内瘤变各组HPV16／18、PC—NA、P^53及ER蛋白阳性表达率与对照组比较差异有统计学意义（P〈0．05）。结论HPV16／18病毒感染、PCNA细胞增殖指数、P^53突变与宫颈浸润性鳞癌、宫颈上皮内瘤变的发生、发展密切相关；HPV16／18、PCNA、P^53的升高与宫颈病变恶性程度呈正相关，ER的升高与其呈负相关。应用原位杂交检测技术与免疫组织化表达有统计学意义可以用于子宫颈病变方面的研究。Objective To discuss the expression of HPV16/18,PCNA,p^53 ,ER in cervix lesion of different degree. Methods FISH was used to detect HPV16/18 and Supervision Two -step Immunohistoehemistry was used to detect the expression of PCNA, p53, ER in 242 eases of squamous carcinoma of cervix, CIN and chronic cervieitis. Result The positive expression rate of HPV16/ 18 ,PCNA,p53 and ER in suqamous carcinoma of cervix group were 96. 77% ,91.94% ,90. 32% ,6.45% respectively, the positive expression rate of HPV16/18 was the highest, the expression of PCNA was strongly high, and the expression high degree CIN were 96. 77%, 100% ,80. 65% respectively , 41.94% ,and the positive expression rate of PCNA was the highest, and shown as strongly high, in low degree CIN, the positive expression rate were 62. 07% ,100% ,62.07%79.31% respectively, and the expression of PCNA was the highest, shown as moderate strong. In control group （Chronic cervieitis group）, only the expression of ER was 100% , the expression of HPV16/18 was only 3.33%, while the expression of PCNA and p53 were both slightly positive and negative. There was significant difference between squamous carcinoma of cervix and high degree CIN （ P 〈 0. 05 ）. Conclusion HPV16/18 virus infection, PCNA cell mitosis index, pS3 mutation were closely associated with the occurrence and development of invasive squamous carcinoma of cervix, CIN. And the increase of ER was correlate with it on the contrary. Thus, FISH and Immunohistochemistry were used totally in the research of the cervix lesion.

关 键 词：HPV16/18 PCNA P^53 ER宫颈鳞癌宫颈上皮内瘤变 

分 类 号：R737.33[医药卫生—肿瘤] R992[医药卫生—临床医学]