胃癌Balb/c小鼠腹膜移植模型建立及生物学特性的研究  

The Establishment and Biologic Characteristic of Transplantable Peritoneal Metastasis of Balb/c Mice

作　　者：徐惠绵 陈峻青 何三光 张淑敏 邢承忠 

出　　处：《中国医科大学学报》1998年第5期490-492,共3页Journal of China Medical University

摘　　要：目的：建立一种能稳定传代的人胃癌小鼠腹膜移植模型。方法：用免疫功能正常的Ｂａｌｂ／ｃ纯系小鼠，经环胞霉素Ａ和环磷酰胺处理，Ｓｙ８６Ｂ裸鼠可移植性细胞株腹腔移植或传代。结果：经１０代观察，移植率（９４．５％）与裸鼠（１００％）接近；但生长潜伏期短，腹水容量大，带瘤生存时间为裸鼠的１倍以上。凡生长癌性腹水的小鼠均发生腹膜广泛转移结节，移植瘤形态学、组织化学、免疫学、分子遗传学及超微结构符合肠型胃癌特点。结论：本模型可作为研究胃癌腹膜转移机理、生物学行为及治疗的动物模型。Objective: Our purpose was to establish a model for transplantable peritoneal metastasis of Balb/c mice. Methods: The Balb/c mice with normal immune function were treated with immunosuppressive agents, Cyclosporin A and Cyclophosphamide. The Sy86B transplantable cell line for nude mice xenografted from human gastric cancer was transplanted and passaged. Results: The transplanted rate in Balb/c mice (94.5%) was colse to that of nude mice (100%). The incubation period was shorter and the volume of ascites was larger than that of nude mice. The survival period of time in Balb/c mice was twice as long as in that of nude mice. Large amount of peritoneal metastatic nodules was found. The morphology, histochemistry, and ultrasructure in xenografted tumor were in accord with the characteristics for intestinal type of gastric cancer. Conclusions: The model could be used to study the mechanism, biologic behavior, provention, or treatment for peritoneal metastasis of gastric cancer.

关 键 词：胃肿瘤 腹膜移植瘤 生物学特性 

分 类 号：R735.2[医药卫生—肿瘤]