加温联合六亚甲基二乙酰胺对MEC-1细胞的作用  

Effects of hyperthermia combined with hexamethylene bisacetamide on mucoepidermoid carcinoma MEC-1 cells

作　　者：王石光[1] 司徒镇强[1] 吴军正[1] 刘斌[1] 王江华[1] 

机构地区：[1]第四军医大学口腔医学院口腔生物学教研室

出　　处：《实用口腔医学杂志》1998年第4期290-291,共2页Journal of Practical Stomatology

摘　　要：目的：研究加温联合六亚甲基二乙酰胺（ＨＭＢＡ）对人粘液表皮样癌ＭＥＣ－１细胞的作用。方法：采用ＭＴＴ法及软琼脂克隆形成法观察加温联合ＨＭＢＡ对ＭＥＣ－１细胞的作用；并用免疫组化法检测加温及联合ＨＭＢＡ对多药耐药（ＭＤＲ）表达蛋白（Ｐ－ｇｐ）的影响。结果：加温联合ＨＭＢＡ对ＭＥＣ－１细胞有协同抑制作用。免疫组化染色显示，对照组及单用药组细胞Ｐ－ｇｐ中度表达；加温组细胞Ｐ－ｇｐ不表达；联合组为低度表达。结论：加温联合ＨＭＢＡ对粘液表皮样癌的治疗可能有一定价值。Objective:To study the effects of hyperthermia combined with hexamethylene bisacetamide(HMBA) on mucoepidermoid carcinoma MEC-1 cells. Methods:MEC-1 cells were treated with HMBA at various concentrations ,or hyperthermia at 42..5℃ for 1h,or of the combination of both.The inhibitory effects were investigated by MTT assay and colonogenic assey respectively.Expression of MDR protin P-gp was measured by immuno-histochemical staining. Results:1)hyperthermia could intensify the effects of HMBA; 2)MEC-1 cells expressed P-gp protein at a medium level , HMBA did not show effects on the expression ,but hyperthermia alone or with HMBA suppressed P-gp expression. Conclusion:Hyperthermia and HMBA may be potential in treating mucoepidermoid carcinoma.

关 键 词：粘液表皮样癌 六亚甲基 二乙酰胺 MEC-细胞 

分 类 号：R739.85[医药卫生—肿瘤]