卵巢肿瘤中CD_(44)变异型的免疫组织化学分析  

Immunohistochemical Study of CD 44 Variant Expression in Human Ovarian Cancers and Its Clinical Implication

作　　者：李巨[1,2] 刘佳[1,2] 鲁海欧[1,2] 江岩[1,2] 郭丽 李宏 

机构地区：[1]中国人民解放军第202医院妇产科 [2]沈阳医学院分子生物学研究室

出　　处：《中国肿瘤临床》1998年第10期738-741,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金;卫生部优秀青年科技人才基金

摘　　要：细胞粘附因子ＣＤ４４的变异型（ＣＤ４４ｖ）出现在多种肿瘤的恶性转化过程中并与这些肿瘤的转移能力密切相关。然而，ＣＤ４４基因在良恶性卵巢肿瘤中的表达类型尚无定论。为此，本研究使用特异性识别ＣＤ４４分子不同抗原决定簇的单克隆抗体，对正常卵巢组织以及良性和恶性肿瘤的ＣＤ４４表达类型进行免疫组织化学分析。结果显示，ＣＤ４４ｖ在正常卵巢内呈阴性；２５例良性肿瘤中，２１例阴性，４例有灶局性变异型外显子ｖ８－ｖ１０的表达；相反，２６例卵巢癌手术切除标本和２株卵巢癌细胞系Ｃａｏｖ３和Ｏｖｃａ３有多种形式的ＣＤ４４ｖ存在；其中，ｖ７的表达明显上调。２例交界性肿瘤的表达形式与卵巢癌相似，但呈灶局阳性。本结果因而提示，ＣＤ４４ｖ尤其是ｖ７出现在卵巢癌恶性转化的过程中；它（们）可能成为该类肿瘤新的生物标志物并在癌细胞转移中起促进作用。Splicing variants of CD 44 molecules (CD 44 v) has been known to be involved in malignant transformation and metastasis of different types of human tumors. However, their status as well as role(s) in ovarian cancers are still in dispute. In this study, CD 44 expression patterns in normal ovarian epithelium,benign ovarian tumors and ovarian cancers were checked immunohistochemically using a series of monoclonal antibodies (MAbs) against the epitopes of standard form of CD 44 (CD 44 s) and CD 44 variant exons respectively. The results revealed that CD 44 v was negative in normal ovarian tissues; 4 out of 25 benign tumors were found with focally expressed variant exons 8 10 (v8 v10), but negative for other exons. In contrast, multiple isoforms of CD 44 v were detected in all of the ovarian cancer cell lines; among those isoforms,v7 was distinctively up regulated and commonly distributed. Our data thus suggest that expressions of CD 44 v especially v7 occur at relatively late stage of stepwised ovarian carcinogenesis which would become a new malignant biomarker and potential immunodiagnostic element for ovarian cancers.

关 键 词：卵巢肿瘤 CD44 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]