血管紧张素Ⅱ对缺氧血管内皮细胞内Ca^(2+)浓度的影响及地尔硫卓的保护作用  

EFFECT OF ANGIOTENSINⅡ ON INTRACELLULAR Ca^(2+) CONCENTRATION OF VASCULAR ENDOTHELIAL CELLS AND PROTECTION OF DILTIAZEM UNDER HYPOXIA

作　　者：马贵喜[1] 李鸣皋[1] 韩磊[1] 黄友章[1] 

机构地区：[1]海军总医院海军航空潜水医学中心,北京100037

出　　处：《解放军预防医学杂志》2009年第5期326-328,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：海军后勤部科研基金项目课题(No.01-3310)

摘　　要：目的观察缺氧条件下血管紧张素Ⅱ(AngⅡ)对人大血管内皮细胞内Ca2+浓度变化的影响,以及地尔硫卓对其的保护作用,为进一步的临床研究提供药理学基础。方法将培养的血管内皮细胞分为4组:对照组、缺氧组、缺氧+AngⅡ组和缺氧+AngⅡ+地尔硫卓组。应用激光共聚焦显微镜测量各组内皮细胞内Ca2+浓度。结果上述4组血管内皮细胞内Ca2+浓度(荧光值)分别为28.46±5.29、40.08±7.80、51.66±8.93、35.49±7.79。缺氧组、缺氧+AngⅡ组细胞内Ca2+浓度明显高于对照组(P<0.01);缺氧+AngⅡ+地尔硫卓组细胞内Ca2+浓度明显低于缺氧组、缺氧+AngⅡ组(P<0.01),接近于对照组(P>0.05)。结论缺氧及AngⅡ可引起血管内皮细胞内Ca2+浓度增加。地尔硫卓能阻止缺氧及AngⅡ引起的内皮细胞内Ca2+浓度升高,对细胞内Ca2+超载具有保护作用。Objective To study the effect of angiotensin Ⅱ （Ang Ⅱ ） and hypoxia on intracellular Ca^2＋ concentration of human vascular endothelial ceils and the protection of Dihiazem, so as to provide pharmacological basis for further clinical study. Methods Cultured vascular endothelial ceils were divided into four groups： （ 1 ） control group, （2） hypoxia group, （3）angiotensin Ⅱ ＋ hypoxia group, （4）Diltiazem ＋ angiotensin Ⅱ ＋ hypoxia group. The changes of intracellular Ca^2＋ concentration were detected with laser scanning confocal microscope. Results （1）The intracellular Ca^2＋ concentration （fluorescene intensity） in hypoxia group, and angiotensin Ⅱ ＋ hypoxia group were significantly higher than that in control group（40.08± 7.8,and 51.66± 8.93 vs 28.46 ±5.29, P 〈 0.01）. （2）The intracellular Ca^2＋ concentration in Diltiazem ＋ angiotensinⅡ ＋ hypoxia group （35.49 ± 7.79） was lower than those in hypoxia group, and angiotensin Ⅱ ＋ hypoxia group （ P 〈 0.01 ）, but not statistically significantly different from that in control group. Conclusion Angiotensin Ⅱ and hypoxia could induce increase in intracellular Ca^2＋ concentration, whereas Diltiazem has significant protection against intracellular Ca^2＋ overload induced by Angiotensin Ⅱ and hypoxia.

关 键 词：地尔硫卓 缺氧 血管紧张素Ⅱ 内皮细胞 钙离子 

分 类 号：R363[医药卫生—病理学]