中缝背核在踏转轮运动昼夜节律和光诱导脑内c-fos表达中的作用  

Role of Dorsal Raphe Nucleus in Wheel-Running Circadian Rhythm and in Photic Induction of c-fos Expression in Brain

作　　者：关宿东[1] 俞光第[1] 蒋星红[1] 许爽[1] 印其章[1] 

机构地区：[1]苏州医学院神经生物学研究室,蚌埠医学院生理学教研室215007

出　　处：《苏州医学院学报》1998年第9期901-904,共4页Acta Academiae Medicinae Suzhou

基　　金：国家自然科学基金!39470240

摘　　要：以小鼠踏转轮运动和大鼠脑内C-fos表达为指标，探讨中缝背核（DR）对下丘脑视交叉上核（SCN）昼夜节律活动的调控机制。在正常光照（LD）和持续黑暗（DD）条件下，电解损毁DR和注射利坦色林后，小鼠踏转轮运动的昼夜节律消失。光照能诱导包括视皮层、梨状皮层、下丘脑室旁核、SCN、膝间体小叶（IGL）、尾壳核、脑桥核和中缝背核在内的广泛脑区的Fos蛋白表达，损毁DR能选择性地减少SCN和IGL中Fos蛋白的表达，对其他脑区影响不大。提示，DR通过SCN胆外侧部和IGL神经元．经特定5－HT受体（可能是5－HT7样受体）.对踏转轮运动的昼夜节律进行调节。For exploring the regulation mechanism of the circadian activity of suprachiasmatic mucleus (SCN ), the role of dorsal raphe mucleus (DR) was investigated in the formation of wheel - running circadian rhythm in mice and in c - fos expression induced by photic stimulation in rat brain. The results showed that under normal light/dark cycle (LD) or constant darkness (DD) condition the wheel - running circadian rhythm in mice disappared after DR lesion, and after ritanserin (5 - HT receptor antagonist) injection as well. Photic stimulation induced widespread expression of Fos protein in different brain areas including visual cortex, pyriform cortex, hypothalamic paraventricular nucleus and SCN, intergeniculate leaflet(IGL), caudate - putamen, nucleus pontis and DR. However, after DR Iesion a significant decrease if light - induced Fos protein expression was observed only in SCN and IGL, while no significant change was detected in other brain areas. The results suggested that the regulation of wheel - running circadian rhythm by DR might be realized in SCN (mainly in ventrolateral part) and IGI, through a specific 5 - HT receptor, Prohably 5 - HT7, like receptor.

关 键 词：视交叉上核 中缝背核 昼夜节律 C-FOS 转轮 

分 类 号：R338[医药卫生—人体生理学]