小儿恶性肿瘤细胞的DNA含量及其临床意义  

The measurement of DNA content in pediatric malignant tumours and its clinical significance

作　　者：刘荻[1] 冯雪莲[1] 蔡玲玲[1] 张致宏 林久治[1] 

机构地区：[1]首都儿科研究所病理研究室

出　　处：《临床与实验病理学杂志》1998年第5期464-466,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：探讨小儿恶性肿瘤ＤＮＡ含量及其与临床预后的关系。方法：用流式细胞仪对６２例小儿恶性肿瘤细胞的ＤＮＡ含量进行测定，并分析其中３５例的细胞增殖状况及其与临床分期的关系。结果：ＤＮＡ组方图呈二倍体的肿瘤１４例，异倍体的肿瘤４８例（检出率７７．４％）；Ⅲ～Ⅳ期肿瘤的异倍体检出率高达９７．４％；ＤＮＡ指数（ＤＩ）和倍性与肿瘤的分型无关。结论：测定结果应与临床、病理资料结合。Purpose To investigate DNA content and its correlation with clinical prognosis. Methods The DNA content of 62 pediatric malignant tumours was determined by flow cytometry. The relationship between S phase fraction(SPF) and clinical stage was analysed in 35 tumours of these cases. Results DNA histograms showed DNA diploidy in 14 cases, aneuploidy in 48 cases (the detectability 77 42%). The detectability of DNA aneuploidy was 97 43% in Ⅲ￣Ⅳ stage. DNA index ( DI ) and DNA ploidy did not correlate with histological type of tumours. Conclusion The prognosis of patients may be exactly estimated by the correlation between the results of DNA content and clinical pathological materials.

关 键 词：儿童 恶性肿瘤 肿瘤细胞 DNA倍体 

分 类 号：R730.21[医药卫生—肿瘤]