胶体金标记单核细胞增生李斯特菌单克隆抗体的制备  

Preparation of Monoclonal Antibodies Against Listeria Monocytogenes Coupled with Colloidal Gold

作　　者：罗雁非[1] 贾芙蓉[2] 白翠华[3] 孔祥云[3] 姜永利[1] 丁旭[1] 何成彦[4] 

机构地区：[1]吉林出入境检验检疫局,吉林长春130021 [2]解放军第208医院 [3]吉林省疾病预防控制中心 [4]吉林大学中日联谊医院

出　　处：《中国卫生工程学》2009年第5期263-265,共3页Chinese Journal of Public Health Engineering

基　　金：国家质检总局计划项目(2006J0313)

摘　　要：目的制备胶体金标记单核细胞增生李斯特菌的单克隆抗体(McAb),用于检测食品中的致病性单核细胞增生李斯特菌。方法以纯化的单核细胞增生李斯特菌(标准菌株号54001、54002)为抗原,免疫Balb/c小鼠,制备单克隆抗体,建立胶体金标记的单核细胞增生李斯特菌单克隆抗体的制备方法。结果筛选出2株单抗杂交瘤细胞株F5、F9,并获得免疫球蛋白,测定免疫球蛋白亚类为IgM。通过选用柠檬酸钠法制备胶体金标记单核细胞增生李斯特菌抗体,测定其最适蛋白结合浓度为12 g/ml。结论成功制备了胶体金标记的单核细胞增生李斯特菌的单克隆抗体,为建立食品中病原性单核细胞增生李斯特菌检测技术奠定基础。Objective To prepare monoclonal antibodies against Listeria monocytogenes which was coupled with colloidal gold , to determine Listeria monocytogenes in food. Methods The Balb/c mice were immunized with the purified Listeria monocytogenes used as an antigen, monoclonal antibodies against Listeria monocytogenes were prepared by normal hybridoma technology, then the colloidal gold was coupled to the Listeria monocytogenes antibodies. Results The immuno - globulin IgM was prepared and the best compatible protein concentration of the colloidal gold was measured as 12 μg/ ml. Conclusion Listeria monocytogenes monoclonal antibodies coupled with colloidal gold have been successfully obtained, so it will build a strong basis for the detection of pathogenic Listeria monocytogenes in the future.

关 键 词：单核细胞增生李斯特菌 单克隆抗体 胶体金 

分 类 号：R155[医药卫生—营养与食品卫生学]