氢醌刺激脐血单个核细胞适应性反应基因表达  

NQO1,HSP10 and 2-Cys Prxs expression in umbilical cord blood mononuclear cells induced by hydroquinone

作　　者：唐深[1] 李习艺[2] 陆彩玲[2] 曾晓春[3] 肖德强[2] 孙斌[2] 郭松超[2] 

机构地区：[1]广西医科大学基础医学院免疫学教研室,广西南宁530021 [2]广西医科大学公共卫生学院 [3]广西医科大学第一附属医院

出　　处：《中国公共卫生》2009年第11期1339-1340,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30600505);广西自然科学基金(0640037);教育部科学技术研究重点项目(208107);广西医科大学博士启动基金(2006)

摘　　要：目的探讨氢醌刺激对脐血单个细胞活性的影响及醌氧化还原酶(NQO1)、热休克蛋白10(HSP10)及巯氧还蛋白过氧化物酶(Prxs)的表达。方法取新鲜脐血分离单个核细胞,10μmol/L氢醌预刺激12 h后,再给予250μmol/L氢醌刺激5 h,磷脂蛋白/溴化丙啶(Anexin V/PI)染色检测细胞活力。荧光定量PCR检测NQO1、HSP10及Prxs的表达。结果脐血单个核细胞受到10μmol/L氢醌预刺激后,可耐受后续250μmol/L氢醌攻击,活细胞数从高剂量组的(44.92±25.10)%提高到(39.37±20.98)%,出现适应性反应。荧光定量PCR结果显示,与空白对照组比较,高剂量组HSP10、Prx3、Prx4表达分别增高(2.22±1.03),(3.44±1.07),(2.97±0.79)倍,适应组Prx1增高(1.08±0.29)倍。NQO1在高剂量组和适应组表达均较空白对照组增高(3.44±1.07)和(3.57±1.31)倍,Prx4在高剂量组表达增高(2.41±0.31)倍,且与适应组比较有明显差别。结论Prx1、Prx3、prx4、HSP10可能参与氢醌诱导的脐血单个核细胞适应性反应。Objective To explore the effect of low dose hydroquinone（HQ） pretreatment on the cell viability and the expression of NQO1,HSP10 and 2-Cys Prxs in umbilical cord blood mononuclear cells （UCBMC）. Methods Mononuclear cell from cord blood were isolated and Annexin V/PI staining assay was applied to evaluate the effect of low level HQ on human hematopoietic system cells. Real time PCR was performed to determine the expression of NQO1, HSP10 and 2-Cys Prxs. Results The results of Annexin V/PI staining assay showed that the UCBMC were more tolerable to 250 μnol/L HQ after pretreated with 10 μmol/L HQ at cell survival level （44. 92 ±25. 10 vs 39. 37 ±20. 98）. The results of real-time PCR showed that compared with the control group, the expression of HSP10, Prx3 and Prx4 significantly increased by 2. 22 ± 1.03,3.44 ± 1.07 and 2. 97 ±0. 79-folds,respectively,in high dose group. The Prxl was increased by 1.08 ±0.29-folds in adaptive group. The expression of NQO1 was increased by 3.44 ± 1.07 and 3.57 ± 1.31-folds, respectively, in high and adaptive group. The expression of Prx4 in high dose group was significantly increased by 2. 41 ± 0. 31-folds compared with that of the control and with a significant difference from that of in adaptive group. Conclusion Prx1, Prx3, Prx4, and Hsp10 may take part in the adaptive response of UCBMC induced by hydroquinone.

关 键 词：氢醌 脐血单个核细胞 NQO1 热休克蛋白10(HSP10) 2-CysPrxs 适应性反应 

分 类 号：R136.3[医药卫生—劳动卫生]