用RAPD技术区分荸荠属中亲缘关系相近的两个种:Eleocharis dulcis和Eleocharis sphacelata(英文)  

Identification of Two Closely related Species of Eleocharis: E dulcis and E sphacelata Using RAPD Analysis

作　　者：李梅[1] 

机构地区：[1]湖北农学院农业工程系

出　　处：《湖北农学院学报》1998年第3期213-217,共5页Journal of Hubei Agricultural College

摘　　要：用ＲＡＰＤ技术鉴别了荸荠属中亲缘关系相近的两个种：Ｅｄｕｌｃｉｓ和Ｅｓｐｈａｃｅｌａｔａ。根据３个１０碱基随机引物（ＳＣ１０２３，ＳＣ１０２５和ＡＢ０１１８）获得的遗传距离值，用ＵＰＧＭＡ聚类方法构建了来自８个样点的样本的分子系统树。在分子系统树中，这８个样点的样本被分为两组：Ｅｄｕｌｃｉｓ和Ｅｓｐｈａｃｅｌａｔａ。Random amplified polymorphic DNA (RAPD) was used to identify two closely related species of Eleocharis:E dulcis and E sphacelata. The use of three decamer primers (SC10-23, SC10-25 and AB01-18) clustered samples from 8 sites into two distinct groups: E dulcis and E sphacelata in a phylogenetic dendrogram obtained by unweighted pair group method analysis (UPGMA) of the genetic dissimilarity. RAPD assay proved to be very powerful for distinguishing these two closely related species.

关 键 词：随机扩增多态性DNA(RAPD) 荸荠 分子系统树 

分 类 号：Q949.714.3[生物学—植物学]