HPLC-ELSD法测定卵磷脂中5种主成分的含量  

Content Determination of 5 Constituents in Lecithin by HPLC-ELSD

作　　者：袁昕蓉 邱志斌 王东凯[2] 

机构地区：[1]沈阳药品快速检验所,沈阳市110013 [2]沈阳药科大学,沈阳市110016

出　　处：《中国药房》2009年第31期2465-2466,共2页China Pharmacy

摘　　要：目的:建立测定卵磷脂中5种主成分磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、神经鞘磷脂(SM)、溶血磷脂酰胆碱(LPC)、溶血磷脂酰乙醇胺(LPE)含量的高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法。方法:色谱柱为YMCPVA-Sil,以氯仿为流动相A、甲醇-水(96:4)为流动相B进行梯度洗脱,流速为0.5mL.min-1,柱温为25℃,检测器为ELSD,参数为漂移管温度40℃、载气(氮气)流量1.0bar。结果:PC、PE、SM、LPC、LPE进样量线性范围分别为2～12、2～12、0.5～3、0.5～3、0.25～1.5μg(r>0.9975);平均回收率分别为99.93%、99.82%、100.02%、99.03%、98.79%(n=9),RSD分别为0.66%、0.42%、0.55%、0.74%、0.98%(n=6)。结论:本法简便、快捷,结果准确可靠,可用于卵磷脂的质量控制。OBJECTIVE： To develop a simple HPLC- ELSD method for the determination of 5 major constituents ineluding phospbatidylcholine（PC）, phosphatidylethanotamine（PE）, sphingomyelin（SM）, lyso phosphatidylcholine（LPC） and lyso- phosphatidytethanolamine（LPE） in lecithin. METHODS： It was separated on YMC PVA- Sil by gradient elution with chloroform as mobile phase A and methanol - water （96 ： 4） as mobile phase B at a flow rate of 0.5 mL ·min ^-1; the column temperature was kept at 25 12 for separation. The detector was ELSD, of which the drift tube temperature was maintained at 40℃ and the flow rate of carrier gas N2 was kept at 1.0 bar. RESULTS： The linear ranges of PC, PE, SM, LPC, and LPE were 2-12, 2-12, 0.5-3, 0.5-3, and 0.25- 1.5 μg, respectively（r〉 0.997 5）; their average recovery rates were 99.93%, 99.82%, 100.02%, 99.03%, and 98.79% （n = 9）, respectively, and their RSD were 0.66%, 0.42%, 0.55%, 0.74%, and 0.98% （n = 6）, respectively. CONCLUSION： The established method is simple, rapid with accurate and reliable results thus applicable for the quality control of lecithin.

关 键 词：卵磷脂 高效液相色谱-蒸发光散射检测器法 含量测定 

分 类 号：R927.2[医药卫生—药学]