(MMTV)-ErbB-2转基因鼠孕期使用三羟异黄酮对子代小鼠乳腺肿瘤中血管内皮生长因子的影响  

Effects of using different doses of genistein during pregnancy on Vascular endothelial growth factor expression in the offsprings of mice mammary tumors of (MMTV)-ErbB-2 transgenic mice

作　　者：张广平[1] 段瑞华[1] 侯建峰[1] 介晓阳[1] 冯笑山[1] 

机构地区：[1]河南科技大学第一附属医院肿瘤科/河南科技大学肿瘤研究所,471003

出　　处：《中国实用医药》2009年第31期3-4,共2页China Practical Medicine

摘　　要：目的观察三羟异黄酮(genistein)对Erb-2转基因小鼠乳腺肿瘤发生的影响。方法采用高剂量:15mg/(kg·d)、中剂量:5.0mg/(kg·d)、低剂量:1.0mg/(kg·d)三羟异黄酮对孕期第13～19天小鼠皮下注射,观察其子代雌性小鼠和对照组子代雌性小鼠乳腺肿瘤的潜伏期和乳腺成瘤率是否存在差异,并用免疫组织化学染色SP方法检测子代小鼠乳腺肿瘤组织VEGF的表达情况。结果高剂量组乳腺癌肿瘤潜伏期、乳腺成瘤率与其它各组存在显著差异性(P<0.05)。高剂量组VEGF的阳性表达水平明显低于其他各组(P<0.05)。结论高剂量异黄酮孕期的使用能够延长子代雌性小鼠肿瘤发生的潜伏期,降低乳腺成瘤率,降低子代小鼠乳腺肿瘤中VEGF的阳性表达水平。Objective This experiment study the effects on the mammary tumors of Erb-2 transgenic mice exposed to genistein Methods The differences in the tumor latency and breast tumor rate between female offsprings of mice of the different doses groups including high-dosel5 mg/（ kg · d） ,middle-dose 5.0 mg/（ kg · d） ,low-dose 1.0 mg/（ kg ·d） of genistein by subcutaneous injection from day 13 to day 19 Of gestation and control groupwere analyzed by statistical methods, the expression of VEGF in the offsprings of mice mammary tumors were detected by immunohistochemistry staining SP method. Results There was a significant difference in breast tumor latency and breast tumor rate between the high-dose group and other groups（ P 〈 0. 05 ）. the positive expression of VEGF levels in the high-dose group were significantly lower than other groups （ P 〈 0. 05 ）. Conclusion Using high-doses of genistein during pregnancy can make the extension of breast tumor latency , the decline of breast tumor rate , the decline of VEGF positive expression levels in the offsprings of mice mammary tumors.

关 键 词：(MMTV)-ErbB-2转基因鼠 乳腺癌 三羟异黄酮 血管内皮生长因子 

分 类 号：R737.9[医药卫生—肿瘤]