大鼠三叉神经节离体培养可用于血管性头痛发病机制和药物治疗的靶点研究  

Organ culture of rat trigeminal ganglion is an ideal in vitro model for pathogenesis study and therapeutic target exploration of primary vascular headache

作　　者：罗国刚[1] 雷莉[2] 吕社民[2] 樊文静[1] 徐仓宝 

机构地区：[1]西安交通大学医学院第一附属医院神经内科,陕西西安710061 [2]西安交通大学医学院遗传学与分子生物学系,陕西西安710061 [3]Division of Experimental Vascular Research, Institute of Clinical Science, Lund University, Sweden 22184, Lund

出　　处：《西安交通大学学报（医学版）》2009年第5期562-566,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30570631);西安交通大学国际交流与合作重点项目(No.0702-07)~~

摘　　要：目的颈动脉血降钙素基因相关肽水平升高是引发血管性头痛发作的重要原因之一,本研究探讨血管性头痛的发生机制和药物作用靶点的理想体外模型。方法SD大鼠三叉神经节在无血清DMEM培养液中离体培养12、24、48 h后免疫组化方法比较降钙素基因相关肽(CGRP)阳性细胞数表达水平,实时定量PCR法(real time-PCR)确定CGRP-mRNA表达水平的变化。结果大鼠三叉神经节离体培养24 h后CGRP的免疫阳性表达细胞数明显增高,CGRP-mRNA表达水平也较新鲜组明显增高(P均<0.05)。结论大鼠三叉神经节离体培养后能精确模拟体内血管性头痛发作期CGRP的变化,是研究血管性头痛发生机制和开发药物作用靶点的理想体外模型。Objective To explore an ideal model for primary vascular headache in studying pathogenesis and new medication therapeutic target in vitro. Methods Trigeminal ganglion （TG） of SD rats was isolated and cultured in serum-free DMEM medium for 12h, 24h and 48h. Then the calcitonin gene-related peptide （CGRP） positive stained cells were detected by using immunohistochemistry staining, and real-time polymerase chain reaction （RT-PCR） was used to determine mRNA expression changes of CGRP. Results The TG cultured in serum-free DMEM for 24 h had a significant increase in the CGRP-positive stained cell number and upregulated strikingly CGRP-mRNA expression compared with fresh TG （P〈0.05）. Conclusion The TG after explant culture with serum-free medium exactly simulates the pivotal change in primary vascular headache attack, which always manifests the increased release of neuropeptides, such as CGRP. Therefore, explant culture of rat TG is an ideal model in vitro for studying pathogenesis and new therapeutic target primary vascular headache.

关 键 词：三叉神经节 离体培养 降钙素基因相关肽 体外模型 

分 类 号：R747.2[医药卫生—神经病学与精神病学]