TLR4参与多发性骨髓瘤细胞增殖及凋亡作用研究  

TLR4 signals are involved in multiple myeloma cell proliferation and apoptosis

作　　者：包悍英[1] 王丽娟[1] 杨杨[1] 蔡真[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2009年第5期465-469,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省卫生厅科学基金(2009A057)

摘　　要：目的:探讨TLR4信号对多发性骨髓瘤细胞增殖及凋亡的影响。方法:用RT-PCR和PE染色流式细胞仪检测骨髓瘤细胞株中TLR4基因的转录和蛋白的表达情况;MTT法检测细胞在脂多糖(LPS)刺激下增殖变化;AnnexinV-PI双染色流式检测细胞经LPS预处理后对阿霉素诱导的细胞凋亡的影响。结果:骨髓瘤细胞株中存在TLR4基因的转录和蛋白的表达,但表达水平有差异。骨髓瘤细胞在LPS刺激下,表达TLR4的MM1-s细胞增殖明显(P<0.05),对阿霉素诱导的凋亡有明显的抵抗作用;而不表达TLR4的U266细胞增殖未受明显影响。而且LPS不能保护阿霉素对U266细胞的诱导凋亡作用。结论:TLR4信号对在多发性骨髓瘤的增殖和生存都起重要作用。Objective： To investigate the TLR4 signaling in multiple myeloma cell proliferation and apoptosis. Methods： TLR4 gene transcription and protein expression were detected by RT- PCR and PE flow cytometry staining; the cells proliferation were detected by MTT assay; doxorubicin-induced apoptosis of cells was detected by AnnexinV-PI double staining flow cytometry. Results： TLR4 gene transcription and protein expression was detected in the myeloma cell lines. Under the lipopolysaccharides （LPS） stimulation, MM1-s cells TLR4 positive proliferation significantly increased （P〈0. 05）, but not found in U266 cells TLR4 negative; MM1-s cells showed the resistance to doxorubisin-induced apoptosis, but LPS did not protect doxorubicin-induced U266 cell apoptosis. Conclusion： TLR4 signaling may play an important role both in multiple myeloma proliferation and survival.

关 键 词：多发性骨髓瘤/病理学 细胞凋亡 脂多糖类 膜糖蛋白类/代谢 受体 细胞表面 细胞增殖 信号传导 细胞 培养的 

分 类 号：R733.3[医药卫生—肿瘤]