Expression Profile of a Novel Germ Cell-specific Gene,TSCPA,in Mice and Human  

作　　者：余振东 吴波 唐爱发 陈静 郭新 秦洁 桂耀庭 蔡志明 

机构地区：[1]Department of Clinical Laboratory,Peking University Shenzhen Hospital [2]Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics,Peking University Shenzhen Hospital

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2009年第5期535-539,共5页华中科技大学学报（医学英德文版）

基　　金：supported by grants from Natural Science Foundation of Guangdong Province,China (No.7008952,No.7300931);National Natural Sciences Foundation of China (No.30700824)

摘　　要：In order to identify novel genes involved in spermatogenesis, testis cDNA samples from Balb/C mice of different postnatal days were hybridized with the whole mouse genome Affymetrix chip to screen the testis-specific genes. The characteristics of the selected genes were analyzed by RT-PCR as well as other bioinformatic tools. A novel differentially expressed testis-specific gene （GenBank Accession No： NM_029042） in the developmental stages of testes was identified, and named TSCPA. Cellular mapping prediction of TSCPA indicated that its protein was probably expressed in nuclei, and one putative domain （aa 332 377） was anchoring domain of cAMP-dependent type Ⅱ PK. The result of subcellular localization of GFP-TSCPA fusion protein in Cos-7 cells showed that TSCPA protein was expressed in nuclei. RT-PCR analysis revealed that TSCPA was expressed specifically in mouse and human testis. TSCPA gene was expressed weakly in 21-day-old mouse testis and the expression was increased gradually from 38th day to 6th month of mouse testes. No expression of hTSCPA was found in cryptorchidism and Sertoli-cell-only syndrome patients. It was concluded that the expression profile of TSCPA in human and mice indicated that TSCPA might play an important role in spermatogenesis.In order to identify novel genes involved in spermatogenesis, testis cDNA samples from Balb/C mice of different postnatal days were hybridized with the whole mouse genome Affymetrix chip to screen the testis-specific genes. The characteristics of the selected genes were analyzed by RT-PCR as well as other bioinformatic tools. A novel differentially expressed testis-specific gene （GenBank Accession No： NM_029042） in the developmental stages of testes was identified, and named TSCPA. Cellular mapping prediction of TSCPA indicated that its protein was probably expressed in nuclei, and one putative domain （aa 332 377） was anchoring domain of cAMP-dependent type Ⅱ PK. The result of subcellular localization of GFP-TSCPA fusion protein in Cos-7 cells showed that TSCPA protein was expressed in nuclei. RT-PCR analysis revealed that TSCPA was expressed specifically in mouse and human testis. TSCPA gene was expressed weakly in 21-day-old mouse testis and the expression was increased gradually from 38th day to 6th month of mouse testes. No expression of hTSCPA was found in cryptorchidism and Sertoli-cell-only syndrome patients. It was concluded that the expression profile of TSCPA in human and mice indicated that TSCPA might play an important role in spermatogenesis.

关 键 词：DNA microarray TSCPA gene SPERMATOGENESIS Sertoli-cell-only syndrome CRYPTORCHIDISM 

分 类 号：R392.1[医药卫生—免疫学]