叶酸对小鼠卵母细胞体外成熟的影响  

Effects of Folic Acid on in Vitro Maturation of Mouse Oocytes

作　　者：黄晓莉[1] 侯绍英[1] 夏蕾[2] 赵艳[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第一医院生殖医学中心,黑龙江哈尔滨150001

出　　处：《癌变．畸变．突变》2009年第5期396-399,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:探讨叶酸(folicacid,FA)对小鼠卵母细胞体外成熟的影响。材料与方法:实验设次黄嘌呤(hypoxanthine,Hx)处理小鼠体外卵母细胞的卵母细胞成熟抑制模型组(Hx4mmol/L+M16培养液)、FA组(500μmol/L FA+4mmol/L Hx+M16培养液)和M16培养液组,各组卵母细胞培养24h后,在体视显微镜下观测卵母细胞生发泡破裂(germinal vesicle breakdown,GVBD)和第一极体(thefirst polar body,PB1)排出情况,采用免疫荧光染色方法观察染色体和纺锤体的形态结构。结果:M16培养液组、Hx模型组和FA处理组的GVBD率分别为98%、22.2%、40.1%,其PB1排出率分别为77.7%、15.5%、27.6%,各组间的差异均具有统计学意义(P<0.05);次黄嘌呤和叶酸对染色体的形态没有影响,而次黄嘌呤使正常的纺锤体变长、变宽、面积变大,FA组较Hx模型组增大的纺锤体有所恢复,并接近于正常水平。结论:叶酸可促进小鼠卵母细胞体外减数分裂的重启和成熟。BACKGROUND AND AIM：To explore the effects of folic acid（FA） on in vitro maturation of mouse oocytes.MATERIALS AND METHODS：Naked mouse oocytes were matured in vitro either spontaneously（M16） or in the presence of hypoxanthine（Hx） or Hx plus FA for 24 h.At the end of the culture period,the nuclear status of mouse oocytes was assessed under inverted microscope.Immunoflurescence staining was used to evaluate the chromosome and spindle configuration of oocytes.RESULTS：The rates of germinal vesicle breakdown（GVBD）were 98% ,22.2% ,40.1% and the rates of the first polar body （PB1） extrusion were 77.7%, 15.5%, 27.6% for M16, Hx and FA groups, respectively. The rates of GVBD and PB1 were all increased with the addition of FA compared with the Hx groups and comparison was significant （P 〈 0.05） Hx and FA did not influence chromosome, but FA reversed the size of metaphase spindle elongated by Hx. CONCLUSION： FA appeared to promote the resumption of meiosis and subsequent maturation of mouse oocytes in vitro.

关 键 词：叶酸 卵母细胞 小鼠 

分 类 号：R730.45[医药卫生—肿瘤]