静脉注射福大赛因对昆明小鼠的皮肤光毒性  

Skin Phototoxicity of Intravenous ZnPcS_2P_2-based-photodynamic Therapy in Kunming Mice

作　　者：李超[1] 陈耐生[2] 魏金锋[1] 薛金萍[2] 沈宁[1] 黄金陵[2] 孙岚[1] 王爱平[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所中国医学科学院中国协和医科大学新药安全评价研究中心,北京100050 [2]福州大学功能材料研究所,福建福州350002

出　　处：《癌变．畸变．突变》2009年第5期400-403,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：科技重大专项"重大新药创制"(2008ZX09305_001)

摘　　要：背景与目的:研究福大赛因对小鼠的皮肤光毒性。材料与方法:将昆明小鼠随机分为5组,分别为生理盐水组(给予生理盐水0.02ml/g),溶剂对照组(给予溶剂0.02ml/g),福大赛因低剂量组(2mg/kg)和高剂量组(5mg/kg)以及阳性对照组(给予血卟啉15mg/kg)。均采用静脉注射1次性给药。小鼠于给药后的不同时间接受不同强度模拟太阳光照射60min。用6mm打孔器取左、右耳廓各1耳片,并称重,通过小鼠耳片重量和肿胀率的变化评价皮肤光毒性。结果:福大赛因高、低剂量组于给药后1、3及24h接受模拟太阳光(40mW/cm2)照射后,耳片重量与对照组相比显著增加(P<0.05),而低剂量组于给药后48h照射后无明显异常,高剂量组则给药后7d经照射无明显异常。给药后1、3h不同强度模拟太阳光照射(10、20、30、40mW/cm2),各组小鼠均出现毒性反应;10mW/cm2组耳片重量与20、30、40mW/cm2组间差异具有统计学意义(P<0.01)。结论:福大赛因2mg/kg体重给药48h后光照无明显光毒性反应;5mg/kg给药7d后光照无明显光毒性反应。福大赛因产生光毒性的饱和光强度约为20mW/cm2。BACKGROUND AND AIM：To investigate the skin phototoxicity of di-sulfo-di-phthalimidomethyl phthalolcyanine zinc（ZnPcS2 P2）-based-photodynamic therapy.MATERIALS AND METHODS：Kunming mice were assigned to five dose groups,receiving intravenous administration of 0.9%NS at 0.2 ml/10 g body weight,the solvent at 0.2 ml/10 g body weight,hemoporphyrin injection at dosage of 15 mg/kg body weight,ZnPcS2 P2 at dosages of 2 or 5 mg/kg body weight.Mice were exposed to simulated sunlight（10,20,30,40 mW/cm^2） 1,3,24,48,72,96 h and 7 d after a single intravenous injection.Skin phototoxicity was investigated by the weight of the slices taken from earflaps by perforex （diameter= 6 mm） . RESULTS： When mice were exposed to light（40 mW/cm^2） 1, 3, 24 h after dosing, there were significant differences of the weight of ears between ZnPcSzP2（2 or 5 mg/kg） and control groups（P 〈 0.05）. There were no significant findings in ZnPcS2P2（2 mg/kg）ZnPcS2P2 group 48 h after dosing, and in ZnPcS2P2（5 mg/kg） group 7 d after dosing.When ZnPcS2P2（2 mg/ kg） group was exposed to light（10, 20, 30, 40 mW/cm^2） 1 or 3 hr after administration, there were significant differences of the weight of ears between 10 mW/cm^2 and 20, 30, 40 mW/cm^2 groups（P〈0.05） . CONCLUSION： In Kunming mice ZnPcS2P2（2 mg/kg） caused phototoxicity 1, 3, 24 h after administration, the potential for phototoxicity was essentially gone after 48 h. Phototoxicity was gone in the skin of mice exposed to simulated sunlight 7 d after injection of ZnPcS2P2（5 mg/kg）. ZnPcS2P2（2 mg/kg） caused skin phototoxicity, the saturated irradiance of the light was 20 mW/cm^2.

关 键 词：光动力治疗 光毒性 光敏剂 

分 类 号：R730.5[医药卫生—肿瘤]