FGFR1特异性拮抗剂SU5402抑制人源晶状体上皮细胞增生的研究  

Inhibitory effect of specific antagonist SU5402 of FGFR1 on growth of human LEC-B3 cells

作　　者：徐庆[1,2] 贾仁兵[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院眼科,200011 [2]上海建工医院眼科,200083

出　　处：《眼科研究》2009年第10期870-873,共4页Chinese Ophthalmic Research

基　　金：上海市卫生局基金资助(034084)

摘　　要：目的本研究探讨体外应用碱性成纤维细胞生长因子(bFGF)受体1(FGFR1)特异性拮抗剂SU5402抑制人源晶状体上皮细胞(LECs)增生的影响。方法光镜下观察传代培养的永生化人源LEC-B3细胞的形态,用αB-晶状体蛋白免疫组织化学法鉴定其性质。采用逆转录聚合酶链反应(RT-PCR)法检测LEC-B3细胞中bFGF和FGFR1的mRNA表达。在传代培养、融合度为70%的LEC-B3细胞中加入SU5402(20μg/mL),以磷酸盐缓冲液(PBS)作为对照,2 d后用流式细胞术(FCM)检测LEC-B3细胞中细胞增生核抗原(PCNA)的表达。结果传代培养的LEC-B3细胞呈多边形上皮样生长,表达特异性的αB-晶状体蛋白;LEC-B3细胞中bFGF和FGFR1的mRNA表达量与内参甘油醛-3-磷酸脱氢酶(GAPDH)相当;LEC-B3细胞中PCNA阳性细胞的比例,SU5402实验组为(23.95±7.82)%,PBS对照组为(58.86±15.43)%,差异有统计学意义(P<0.01)。结论LEC-B3细胞丰富表达bFGF和FGFR1的mRNA;体外应用SU5402可显著抑制LEC-B3增生,有望成为防治后囊膜混浊(PCO)的有效方法。Objective Posterior capsule opacification （PCO） is a common complication after cataract surgery and its underlying mechanism remains below understanding. Basic fibroblast growth factor （bFGF） is thought to play a role in the development of PCO. This study was designed to explore the effect of SU5402, a specific antagonist of bFGF receptor 1 （ FGFR1 ） , on cultured human lens epithelial cells （LECs）. Methods LEC-B3 cells derived from human LECs were cultured in DMEM with 10% fetal bovine serum. Microscope-based evaluation and immunohistochemistry of αB-crystal protein were used to identify LEC-B3 cells derivation. Reverse transcription polymerase chain reaction （RT-PCR） was performed to detect the mRNA expression of bFGF and FGFR1 in cultured cells. SU5402 at the concentration of 20 μg/mL was added into cultured LEC-B3 cells at confusion of 70% and phosphate buffer solution （PBS） was used as negative control. Proliferation cell nuclear antigen （PCNA） expression was examined by flow cytometry （FCM） 2 days later after administration of SU5402. Results Cultured LEC-B3 cells were found to grow as epithelialdike cells with the polygonal shape. Specific αB-erystal protein was strongly expressed in cytoplasm of LEC-B3 cells. The mRNA level of bFGF and FGFRI in LEC-B3 cells was comparable to that of control （ GAPDH ）. The percentage of PCNA expression in LEC-B3 cells in SU5402 treated group was significantly lower than that in PBS treated group （23.95%±7.82% versus 58.86% ±15.43%） （t=6.374,P〈0. 01）, Conclusion SU5402 inhibits the growth of LEC-B3 cells ex vivo,which promise to be an effective way to deal with posterior capsule opacification（PCO）.

关 键 词：后囊膜混浊 碱性成纤维细胞生长因子 成纤维细胞生长因子受体 晶状体上皮细胞-B3 成纤维细胞生长因子受体拮抗剂 SU5402 

分 类 号：R776.1[医药卫生—眼科]