线粒体融合素基因-2对乳腺癌细胞RECK基因表达的影响  

Effect of Mitofusin-2 Gene on RECK in Breast Carcinoma Cell

作　　者：张勇[1] 郑启昌[1] 王尧[1] 胡文君[1] 胡青钢[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022

出　　处：《肿瘤防治研究》2009年第10期818-820,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30700797)

摘　　要：目的探讨线粒体融合素基因-2(mfn2)对乳腺癌细胞株MCF-7细胞中RECK表达的影响。方法利用脂质体lipofectamine2000将构建的重组真核表达质粒pEGFP-mfn2转染MCF-7细胞。RT-PCR检测细胞mfn2和RECK基因mRNA的转录水平;Weastern blot法检测mfn2及RECK蛋白的表达。结果转染pEGFP-mfn2质粒的MCF-7细胞可以稳定高表达mfn2,RECK基因在转染空质粒组和未转染组细胞中无表达,转染mfn2基因后RECK基因mRNA转录及蛋白的表达显著升高。结论mfn2基因可激活MCF-7细胞中RECK基因的表达。Objective To investigate the effect of mfn2 gene on expresssion of RECK gene in breast carcinoma cell（MCF-7）.Methods The pEGFP-mfn2 was transfected to MCF-7 with lipofectamine2000,the mRNA levels of mfn2 and RECK were detected by RT-PCR;Western blot analysis was used to detect the protein expression of mfn2 and RECK gene.Results After transfected by pEGFP-mfn2,the stable expression of Mitofusin-2 gene was detected in MCF-7 cells,the expression of RECK mRNA and protein were significantly activated in transfected Mitofusin-2 gene group,but not detected in transfected pEGFP and non-transfected cells.Conclusion The mfn2 gene can activate the expression of RECK in MCF-7.

关 键 词：线粒体融合素基因-2 MCF-7 RECK 

分 类 号：R737.9[医药卫生—肿瘤]