MTT染色法检测化疗后骨肉瘤细胞的凋亡  

MTT assay for detecting osteosarcoma cell apoptosis

作　　者：陈强[1] 张进华[2] 陈建庭[1] 史占军[1] 邓永健[2] 

机构地区：[1]南方医科大学南方医院脊柱骨病外科,广东广州510515 [2]南方医科大学南方医院病理科,广东广州510515

出　　处：《南方医科大学学报》2009年第9期1899-1901,共3页Journal of Southern Medical University

基　　金：南方医院院长基金(20060009)

摘　　要：目的研究MTT染色法用于检测与区分化疗后活的与凋亡的骨肉瘤细胞可行性。方法以手术切取的骨肉瘤组织的细胞建立凋亡细胞模型,采用MTT染色法检测,直接在光镜下进行形态学观察,并与荧光染色方法的观察结果进行比较。结果MTT染色法可准确判定化疗后活的与凋亡的骨肉瘤细胞,并可清晰分辨正常细胞、凋亡细胞和死亡细胞。结论MTT染色法是一种简单、可行的检测骨肉瘤细胞凋亡的方法,可用于化疗后肿瘤坏死率的研究。Objective To assess the feasibility of using MTT assay for detecting the apoptosis ofosteosarcoma cells following chemotherapy. Methods The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope. Results MTT staining accurately identified apoptosis ofthe osteosareoma cells, and the apoptotic cells were easily distinguished from normal ceils and dead cells. Conclusion MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.

关 键 词：骨肉瘤细胞 细胞凋亡 MTT染色 荧光染色 

分 类 号：R738.1[医药卫生—肿瘤]