大鼠脑梗死后乏氧组织变化与血管增生的关系  

Relationship between change of hypoxic tissue and vascular proliferation after cerebral infarction in rats

作　　者：潘经锐[1] 王艺东[1] 李梅[1] 吕瑞妍[1] 邱宇[1] 彭英[1] 

机构地区：[1]中山大学附属第二医院神经科,广东广州510120

出　　处：《中国病理生理杂志》2009年第10期1926-1930,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技计划资助项目(No.2007B031502007)

摘　　要：目的:探索脑梗死后乏氧组织的变化与血管增生的关系。方法:利用大鼠脑缺血1.5h再灌注(1.5hIR组)和持续缺血(PI组)的线栓模型,采用2-(2-硝基-1氢-硝基咪唑-1-羟基)-氮-(2,2,3,3,3-5氟嘌呤)乙酰唑胺(EF5)和血管性假性血友病因子(vWF)免疫荧光方法观察乏氧组织和微血管密度(MVD),比较2组术后3d、7d、14d缺血侧大脑半球皮层的MVD以及与乏氧组织的关系。结果:1.5hIR组术后3d、7d、14d均见乏氧组织存在,PI组乏氧组织仅存在3d,假手术组不存在乏氧组织。各观察时点的乏氧组织内部MVD均较周围区域的MVD小(均P<0.01)。随着时间的延长,1.5hIR组和PI组的皮层MVD均不断增大(均P<0.01),而1.5hIR组7d和14d的皮层MVD均高于PI组(均P<0.01)。结论:脑梗死后乏氧组织出现在微血管稀疏的区域。微血管增生程度的差异是导致乏氧组织持续时间不同的原因之一。AIM： To clarify the relationship between the change of hypoxic tissue and vascular proliferation after cerebral infarction. METHODS ： Rats with middle cerebral artery occlusion were randomly divided into 1.5 h ischemia -reperfusion （1.5 h IR） group and permanent ischemia （PI） group. Double staining by immunofluorescence was used to identify the hypoxic tissue and microvessels by EF5 and vWF labelings, respectively. On 3 d, 7 d and 14 d post - operation, microvessel density （MVD） and hypoxic tissue in isehemic cortex were observed. RESULTS： Hypoxic tissues existed from 3 d to 14 d post - operation in 1.5 h IR group and disappeared after 3 d in PI group. The MVD within hypoxic tissue was smaller than that around it at all observed time points （ all P 〈 0. 01 ）. With the lapse of time, MVD in ischemic cortex gradually increased both in 1.5 IR and PI groups （ all P 〈 0. 01 ） and was bigger in 1.5 h IR group than that in PI group on 7 d and 14 d （ both P 〈 0. 01 ）. CONCLUSION ： Hypoxic tissues mainly locate in areas of sparse microvessel after cerebral infarction and their duration is partially dependent on the grade of microvessel proliferation in ischemic cortex.

关 键 词：脑梗死 缺氧 微血管密度 免疫荧光 

分 类 号：R743[医药卫生—神经病学与精神病学]