传代培养在羊水产前诊断中的应用  

To study on the methods of serial ubcultivation of amniotic fluid in penatal diagnosis

作　　者：李锋[1] 汪明[1] 

机构地区：[1]武汉大学人民医院检验科,430060

出　　处：《中国优生与遗传杂志》2009年第10期47-48,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的建立一种成功率高,收获分裂相多的羊水细胞培养方法。方法总共83例羊水标本分成两组分别用经典培养法和传代培养法培养。结果经典培养成功率为90.6%(39/43),传代培养成功率为95%(38/40),传代培养方法成功率高于经典培养方法,且所获分裂相数目多,形态好。结论传代培养方法是一种简便易行的羊水细胞培养方法。Objective ： The aim of this study is to establish an amniotic fluid cultivation method of high successful rate. Methods ： The classic cultivation method was performed in 43 cases of amniotic fluid cultivation and serial subcultivation method was performed in 40 cases of amniotic fluid cultivation. Results ： The successful rate of classic cultivation method and serial subcultivation method was 90. 4% （39/43） and 95% （38/40） respectively. The serial subcultivation has high successful rate than the classics culture. More and high - quality M - phase can be observed by serial subcuhivation method. Conclusion： The serial subcuhivation is convenient method in amniotic fluid culture. It has the advantages of good chromosome morphous and high successful rate.

关 键 词：羊水 细胞培养 产前诊断 

分 类 号：R714.55[医药卫生—妇产科学]