洗涤剂用碱性纤维素酶的研究(Ⅱ)——酶的提取及性质  

Alkaline Cellulase for Laundry Detergent (Ⅱ) ——The Extraction and Properties of Alkaline Cellulase

作　　者：余晓斌[1] 施炜[1] 全文海[1] 章克昌[1] 

机构地区：[1]无锡轻工大学生物工程学院

出　　处：《工业微生物》1998年第2期10-14,共5页Industrial Microbiology

基　　金：国家"八五"科技攻关项目

摘　　要：产碱性纤维素酶的嗜碱芽孢杆菌ＳＨＹ８－５７２５发酵液经絮凝预处理后，采用混合盐析方法沉淀酶，盐析收率可达９５％以上，所制得酶粉ＣＭＣａｓｅ可达５００ｕ／ｇ以上。酶的最适ｐＨ为８．０和１０．５，稳定ｐＨ范围为４～１２；最适温度为４５°Ｃ（ｐＨ９．０，１０ｍｉｎ），稳定温度范围为５０°Ｃ（ｐＨ９．０，３０ｍｉｎ）以下；洗衣粉中各种表面活性剂和助剂对ＣＭＣａｓｅ基本无影响；酶的底物特异性表明，该碱性纤维素酶主要为内切型酶。Abstract Alkaline cellulase was produced by alkalophilic Bacillus SHY8 5725. The fermented broth was pretreated by flocculant, then enzyme was precipitated by the addition of mixed salt. The recovery of enzyme activity with salt extraction was over 95% and the CMCase activity of enzyme powder was over 500 u/g. The optimum pH of prepared enzyme was 8.0 and 10.5, working ranges 4-12; optimum temperature was 45°C and its activity was stable up to 50°C. CMCase activity was not affected by various surfactants, builders used in laundry detergent. The substrate specificity showed that the alkaline cellulase was an endo type cellulase.

关 键 词：碱性 盐析 内切型 纤维素酶 提取 洗涤剂用酶 

分 类 号：TQ649.64[化学工程—精细化工] Q556.2[生物学—生物化学]