微量法快速提取甘薯叶DNA条件的优化  

Optimization on the Conditions for Rapid Extraction of DNA from Sweetpotato Leaves by Micromethod

作　　者：吴列洪[1] 谢德平[2,3] 张燕春[4] 陆国权[5] 

机构地区：[1]浙江省农业科学院,浙江杭州310021 [2]浙江大学农业与生物技术学院,浙江杭州310029 [3]西南大学农学与生物科技学院,重庆400716 [4]浙江师范大学化学与生命科学学院,浙江金华321004 [5]浙江林学院薯类作物研究所,浙江临安311300

出　　处：《安徽农业科学》2009年第31期15157-15159,共3页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑项目(2006BAD01A06-2);国家公益性行业项目(nyhyzx07-012-12);浙江省科技厅重大项目(2007C12079)

摘　　要：[目的]筛选出一种简便快捷的DNA微量提取方法。[方法]从不同处理甘薯植株的叶片中提取DNA样品并用紫外分光光度法和琼脂糖凝胶电泳法检测其纯度和质量。[结果]正常光照下甘薯植株的DNA产量最高,48 h黑暗处理的次之,24 h黑暗处理的最低。在正常光照下和48 h黑暗处理中顶芽的DNA产量都明显高于叶片;在24 h黑暗处理中,顶芽与叶片的DNA产量比较接近。叶片DNA的纯度高于顶芽,正常光照下和24 h黑暗处理中DNA的纯度高于48 h黑暗处理中的。DNA电泳图谱表明,正常光照下叶片DNA的电泳条带一般比顶芽的好。48 h黑暗处理中顶芽和叶片DNA的电泳条带没有很大差异。[结论]该试验为大批量实生苗DNA的快速提取和多种分子生物学研究奠定了实践基础。[ Objective ] The aim of the study was to screen out a simple and rapid micro-extraction method of DNA. [ Method ] The DNA samples were extracted from the leaves of sweetpotato plants in different treatments and their purities and qualities were detected by UV speetropho- tometry and agarose gel electrophoresis. [ Result ] The yield of DNA from the sweetpotato plants growing under normal light was highest, that in the dark treatment for 48 h was secondary and that in the dark treatment for 24 h was lowest. Under normal light and in the dark treatment for 48 h, the yield of DNA from apical buds was obviously higher than that from leaves. In the dark treatment for 24 h,the yields of DNA from apical buds and leaves were relatively close. The purity of DNA from leaves was higher than＇that of DNA from apical buds. The purities of DNA under normal light and in the dark treatment for 24 were higher than that in the dark treatment for 48 h. The electrophoretogram of DNA showed that the electro- phoretic bands of DNA from leaves growing under normal light were better than that of DNA from apical buds generally. In the dark treatment for 48 h, the electrophoretic bands of DNA from apical buds and leaves had no great difference. [ Conclusion ] This experiment laid a practical foundation for the rapid extraction of DNA from mass seedlings and many molecular biology studies.

关 键 词：甘薯 DNA提取 叶 电泳 

分 类 号：S531[农业科学—作物学]