4种卷柏类药材中2种特征性成分研究  

Studies on Two Characteristic Constituents in Four Species of Herbs Selaginella

作　　者：徐康平[1] 申健[1] 刘建锋[1] 李福双[1] 杨帆[1] 刘国如[1] 李拓颖[1] 谭桂山[1] 

机构地区：[1]中南大学药学院,长沙410013

出　　处：《中国药学杂志》2009年第20期1535-1539,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30873149);湖南省科技厅科技计划资助项目(2008FJ4182);教育部"大学生创新性实验计划"资助项目(YA09060);中南大学贵重仪器开放共享基金项目(ZKJ2009020)

摘　　要：目的在对4种卷柏类药材中2种特征性成分selaginellin和穗花杉双黄酮进行结构确证的基础上,首次建立同时测定此2种成分含量的HPLC方法。方法采用岛津VP-ODS色谱柱(4.6mm×250mm,5μm),流动相为乙腈-0.2%醋酸水溶液,梯度洗脱,流速为1.2mL·min-1,检测波长为432和338nm,柱温为30℃。结果在此色谱条件下,selaginellin和穗花杉双黄酮得到完全分离,分别在0.025～1.016和0.221～8.853μg内线性良好(r均为1.0000),平均回收率分别为98.64%和99.34%,RSD分别为0.86%和0.94%(n=6)。结论Selaginellin为首次从垫状卷柏中分离得到。含量分析方法操作简单、快速、准确,灵敏度高,重现性好,可为卷柏类药材质量评价和控制提供参考;对4种卷柏类药材进行了测定,所测样品含量差异较大。OBJECTIVE To establish a HPLC method for the quantification of selaginellin and amentoflavone in four species of herbs Selaginella, which were two characteristic constituents in Selaginella on the basis of structure elucidation. METHODS The determination was performed on a Shimadzu VP-ODS column （4.6 mm×250 mm, 5 μm）. Acetonitrile -0.2% acetic acid solution was used as the mobile phase with a gradient elution. The flow rate was 1.2 mL.min-1. The UV detection wavelength was at 432 and 338 nm. The column oven temperature was at 30 ℃. RESULTS Selaginellin and amentoflavone were separated, and remained in good linearity over the ranges of 0.025-1.016 pg and 0.221 8.853 respectively（r=1.000 0）. The average recoveries were 98.64% and 99.34% , and the RSDs were 0.86% and 0.94% （n 6）. CONCLUSION： Selaginellin was isolated from Selaginella pulvinata（Hook et Grev.） Maxim for the first time. The method is accurate, simple and reproducible, and can be used to control the quality of herbs Selaginella.

关 键 词：卷柏 selaginellin 穗花杉双黄酮 结构确证 含量测定 高效液相色谱法 

分 类 号：R282[医药卫生—中药学]