黄鳝二价体上微量DNaseⅠ敏感性和限制性内切酶原位切割分析  

A Study of Pachytene Bivalents in Rice-field Eel Using DNase Ⅰ-hypersensitivity and Restriction Endonuclease-directed in situ Nick Translation

作　　者：杜丽萍[1] 余其兴[1] 周荣家[1] 崔建勋[1] 郭一清[1] 赵则春[1] 

机构地区：[1]武汉大学生命科学学院

出　　处：《Acta Genetica Sinica》1998年第4期308-314,共7页

基　　金：国家自然科学基金~~

摘　　要：采用DNase Ⅰ超敏感性分析和限制性内切酶介导的原位切口平移技术(Restriction Enzyme Nick Translation,RE-NT)对黄鳝二价体基因组结构进行了分析研究。已知DNaseⅠ超敏感性与潜在活性基因分布密切相关。结果表明,经DNaseⅠ介导的原位切口平移处理,在黄鳍二价体上可展现类D带带型,而由限制性内切酶介导的原位切口平移结果显示,AluⅠ和MspⅠ均在黄鳝二价体上诱导产生类G带带型,HpaⅡ和HaeⅢ则优先切割5号二价体上一特定区域,诱导出一段由标记信号所构成的类C带,对上述结果进行了分析和讨论。We had studied the genome organization on pachytene bivalents in Rice-field Eel,using two methods, DNase Ⅰ-hypersensitivity and restriction endonuclease-directed insitu nick translation. DNase Ⅰ hypersensitivity is known to be associated with potentiallyactive genes, When the reaction was detected by DNase Ⅰ in situ nick translation, aD-like banding pattern was produced on pachytene bivalents of Rice-field Eel. Withthe treat of REs-directed in situ nick translation, Msp Ⅰ and Alu Ⅰ were able toinduce G-like banding patterns, and DNA Cleavage by HpaⅡ and HaeⅢ occuredprefemtially in a special region of bivalent 5, in which a unique C-like banding patternwas shown. The results were analysed and discussed in this paper.

关 键 词：原位切口平移 DNASEI 限制性内切酶 黄鳝 二价体 

分 类 号：Q783[生物学—分子生物学]