紫外分光光度法测定芦柑中的还原型维生素C  

Determination of Ascorbic Acid in Orange by Ultraviolet Spectrophotometry

作　　者：蔡顺香[1] 

机构地区：[1]福建省农业科学院土壤肥料研究所,福州市晋安区新店镇埔垱村350013

出　　处：《光谱实验室》2009年第5期1091-1094,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：研究紫外分光光度法测定芦柑中还原型维生素C的新方法。利用活性炭将还原型维生素D氧化为在紫外区无吸收的脱氢抗坏血酸,测定氧化前后的OD_(245)之差,得出芦柑中还原型维生素C的含量。该方法简单、快速、准确性及精密度好,与2,6-二氯靛酚滴定法比较,结果基本一致。A new method was established to determine the ascorbic acid in orange based on oxidation of the reduction type Vc by active carbon.The contents of ascorbic acid in orange was obtained by determination of the different OD value at 245nm.The method was simple,quick and accurate.The results was the same as the method of 2,6-dichlorophenolindophenol titration.

关 键 词：紫外分光光度法 芦柑 维生素C 抗坏血酸 

分 类 号：O657.32[理学—分析化学]