不同时间窗电针治疗对脑梗死大鼠Bcl-2和BDNF表达的影响  

Effect of electro-acupuncture in different time windows on the expression of Bcl-2 and brain derived neurotrophic factor in rats with cerebral infarction

作　　者：李嫚[1] 王梦嵽[1] 梅元武[1] 黎刚[1] 方瑗[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,湖北武汉430022

出　　处：《中风与神经疾病杂志》2009年第5期603-605,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助(项目批准号:30640010)

摘　　要：目的研究大鼠脑缺血-再灌注后不同时间窗开始电针(electroacupuncture)治疗对其康复的影响。方法将100只SD大鼠随机分为正常组、假手术组、电针组和模型组,制备左侧大脑中动脉栓塞/再灌注模型.电针组于动物再灌注后不同时间窗给予电针治疗,于治疗14d后取脑,采用RT-PCR检测梗死侧Bcl-2mRNA、BDNFmRNA的表达。结果各组均检测到BDNF mRNA和Bcl-2mRNA阳性表达,在电针12h亚组可以检测到BDNFmRNA的表达明显高于其它各组,有显著性差异(P<0.05);在电针24h组可以检测到Bcl-2mRNA的表达明显高于其它各组,有显著性差异(P<0.05)。结论在12h^24h时间窗给予电针治疗能促进大鼠脑梗死后梗死侧BD-NF mRNA和Bcl-2mRNA的表达达到峰值,从而发挥其脑保护及康复治疗的最大效应。Objective To study the effect of electro-acupuncture in different time windows on the rehabilitation of rats with cerebral infarction. Methods One hundred SD rats were randomly divided into four groups：normal group （n = 25 ）, sham-operated control group （ n = 25 ）, model group （ n = 25 ） and electro-acupuncture group（ n = 25 ）. The cerebral infarction model was established by left middle cerebral artery occlusion（MCAO）. Electro-acupuncture was started at different time points after reperfusion, and the expression of Bcl-2 mRNA and BDNF mRNA was measured by RT-PCR at 14d after therapy,respectively. Results The positive expression of Bcl-2 mRNA and BDNF mRNA was detected in the brain around the infarction area in all groups. The expression of Bcl-2 mRNA was the highest in the electro-acupuncture 24h group, and that of BDNF mRNA in the electro-acupuncture 12h group in comparison with all the other groups（ P 〈 0.05 ）, respectively. Conclusion The expression of Bcl-2 mRNA and BDNF mRNA in the brain of rats with cerebral infarction would reach the peak when electro-acupuncture was administered in the 12 - 24h time window after reperfusion,and electro- acupuncture might have the maximal protective and rehabilitative effects on rats with cerebral infarction.

关 键 词：电针 脑梗死 细胞淋巴瘤/白血病-2基因 脑源性神经营养因子 

分 类 号：R743.3[医药卫生—神经病学与精神病学]