绿色木霉原生质体的激光诱变及纤维素酶发酵条件优化  

Laser mutagenesis and producing cellulase condition optimization of Trichoderma virid protoplast

作　　者：陈淑丽[1] 张琴[1] 韩晶晶[1] 吕江涛[1] 汪世龙[1] 姚思德[2] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]中国科学院上海应用物理研究所,上海201800

出　　处：《核技术》2009年第11期828-832,共5页Nuclear Techniques

基　　金：国家自然科学基金(50673078);上海市教委科技创新项目(08ZZ21)资助

摘　　要：采用Nd:掺钕的钇铝石榴石固体激光器,在266nm激发波长下,对绿色木霉CICC13038原生质体进行辐照诱变,通过微晶纤维素筛选,得到高产纤维素酶的菌株JG13,运用正交试验对诱变菌株发酵条件进一步优化,在28℃、180r/min、发酵72h条件下的正交试验结果表明:发酵条件为1%(NH4)2SO4为氮源,2%麸皮为碳源,0.5%Tween-80为产酶促进剂,瓶装量25mL(250mL发酵瓶),纤维素酶酶活可达35.68U/mL,相同发酵条件下,较原始菌株酶活提高25.76%。菌株JG13也可作为进一步诱变筛选的出发菌株,获得更高活性菌株。The protoplast of Trichoderma virid CICC13038 was mutated using Nd：YAG laser of 266 nm light. And a high-cellulase producing strain JG13 was bred by screening with cellulose microcrystalline. Under the condition of 28℃, 180 rpm and 72 h of fermentation time, optimal conditions for the celluase ferment by orthogonal experiment were： 2% bran as the carbon source, 1% （NH4）2SO4 as the nitrogen source, 0.5% Tween-80 as a enzyme-promoting agent, and 25 mL of medium volume in a 250 mL bottle. The cellulase activity of the mutant reached 35.68 U/mL, 25.76% higher than that of the original strain under the same conditions. The mutant JG13 has a great potential in industrial production. And it also can be used as the original strain for further mutagenesis to get the strain of higher cellulase activity.

关 键 词：绿色木霉 原生质体 激光诱变 纤维素酶 

分 类 号：Q631[生物学—生物物理学] Q345.1