糠醛渣的纤维素酶水解及其最优纤维素转化条件  

Enzymatic hydrolysis of corncob residues of furfural manufacture and optimum conditions for cellulose conversion

作　　者：仉磊[1] 李涛[2] 王磊[3] 李十中[1] 

机构地区：[1]清华大学核能与新能源技术研究院,北京100084 [2]天津科技大学教育部工业微生物重点实验室,天津300457 [3]河南农业大学生命科学学院,郑州450002

出　　处：《农业工程学报》2009年第10期226-230,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金："十一五"国家科技支撑计划重大项目(2006BAD07A01)

摘　　要：该文对糠醛渣的纤维素酶水解特性进行了研究,探索利用玉米芯制糠醛联产燃料乙醇工业化生产的可行性。分析糠醛渣组分,表明其半纤维素质量分数为3.1%,纤维素为31.6%,说明糠醛生产过程对玉米芯的预处理基本满足高效酶解糖化糠醛渣并转化乙醇的要求;通过纤维素酶用量、温度、pH值、固液比、转速等因素进行条件优化,确定最佳水解条件:每克底物酶用量为6.7FPU,固液质量体积比1︰6,pH5.2,转速80r/min;在糠醛渣水解体系中加入吐温80,结果表明在酶施用量较低情况下(6.7FPU/g),吐温80对提高糠醛渣水解转化率效果更为明显;通过最优化水解条件,使糠醛渣纤维素转化率达到78%,据此初步判定以糠醛渣为原料转化乙醇的工业化生产具有较大潜力。Saccharification of lignocellulose is a key cost center in the overall bioconversion process. Enzymatic hydrolysis of corncob residues of furfural manufacture（CRFM） was investigated to study the feasibility of mass production of ethanol. Compositional analysis results showed that 3.1% of hemicellulose and 31.6% of cellulose existed in CRFM, which demonstrated that the furfural production process could be regarded as pretreatment of lignocellulose for consequent bioconversion. Parameter optimization of hydrolysis was performed and optimal conditions were as follows： 6.7 FPU of enzymes loading per gram substrate, solid-liquid ratio of 1：6, pH5.2, shaking at 80 r/min. The addition of Tween 80 had more positive effect on enzymatic hydrolysis of CRFM with comparatively low enzyme loading （6.7 FPU/g） than the higher one （10 FPU/g）. Cellulose conversion of CRFM hydrolysis was promoted up to 78% under the optimal conditions mentioned above. So co-production of ethanol with CRFM may be a potential way for the industrialized production of ethanol.

关 键 词：水解 乙醇 纤维素 糠醛渣 

分 类 号：S216.2[农业科学—农业机械化工程]