一种可控的切应力及LDL浓度极化动物模型的建立及对动脉粥样硬化的影响  

Establishment of controllable wall shear stress and LDL concentration polarization model in rabbits and their effect on atherosclerosis

作　　者：刘华[1] 王贵学[1] 叶林奇[1] 李培培[1] 向成平[1] 何静[2] 赵禾[2] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]重庆大学医院,重庆400044

出　　处：《第三军医大学学报》2009年第20期1981-1985,共5页Journal of Third Military Medical University

基　　金：教育部科学技术研究重点项目(104158);重庆大学2007年国家大学生创新性实验项目(CQUCX-G-2007-03)~~

摘　　要：目的建立一种可调控的兔颈动脉切应力及低密度脂蛋白(low density lipoprotein,LDL)浓度极化动物模型,初步观察二者对动脉粥样硬化(artherosclerosis,AS)形成的影响。方法兔左颈总动脉施以3个狭窄度(30%、40%、50%)的硅胶管环扎手术,以右颈总动脉为假手术对照;饲喂含4种不同胆固醇浓度(0%、0.5%、1%、1.5%)饲料;彩色超声检测双侧颈总动脉血流动力学参数,计算切应力值,血管标本行病理及扫描电镜观察。结果同一狭窄度,左颈总动脉切应力随饲喂饲料所含胆固醇浓度的增加而增加;同一胆固醇浓度,左颈总动脉切应力以40%狭窄度组最低;同一样本,左颈总动脉切应力高于右颈总动脉(P<0.05)。病理切片及扫描电镜检测显示各组动物左颈总动脉均出现不同程度的AS病变,以40%狭窄度组病变更为显著。结论成功建立了可调控的兔颈总动脉切应力及LDL浓度极化模型,低切应力及LDL浓度极化促进AS的形成和发展。Objective To establish a controllable wall shear stress （WSS） and low density lipoprotein （LDL） concentration polarization animal model to investigate the effects of these 2 factors on the formation of artherosclerosis （AS） in vivo. Methods The left common carotid arteries （LCCA） of rabbits were ligated by a gel-silica pipe loop ligature with 3 different stenosis degree （30%, 40% or 50%） to generate different blood flow environment on local blood vessel, while the right common carotid artery （RCCA） was performed as a sham operation control. After operation the animals were fed with 4 kinds of forages with different cholesterol concentration （0%, 0.5%, 1% or 1.5%） to form different level of LDL concentration polarization. Hemodynamics parameters of bilateral common carotid artery （CCA） were measured by color Doppler flow imaging at 8 weeks postoperatively, then the animals were killed to get bilateral CCA examples for pathological observation and scanning electron microscopy （SEM）. Results In the case with same stenosis degree, WSS of LCCA was increased with the augmentation of cholesterol concentration. In the case of same cholesterol concentration, WSS of LCCA in 40% stenosis degree group was the lowest. In the case of same sample, WSS of LCCA was higher than RCCA （P0.05）. The observation of pathological section and SEM indicated there were atherosclerotic lesions in different extent in LCCA of all groups, but the lesions of 40% stenosis degree were most significant. Conclusion A controllable WSS and LDL concentration polarization model of CCA in rabbit is established successfully, and low WSS and LDL concentration polarization contribute to the formation and development of AS.

关 键 词：动脉粥样硬化 切应力 低密度脂蛋白 浓度极化 动物模型 

分 类 号：R-332[医药卫生] R543.5