氧调节蛋白150在人肝细胞癌中的过度表达及其对凋亡的作用(英文)  

Overexpression and Effect on Apoptosis of the 150-ku Oxygen-regulated Protein(ORP150) in Human Hepatocellular Carcinoma

作　　者：周海军[1,2] 黑振宇[1] 史炯[1] 郭坤[1,2] 孙冰生[1] 武金才[1] 赵越[1] 付丽芸[1] 戴春[1] 高东梅[1] 孙瑞霞[1] 赵燕[1] 陈洁[1] 王鲁[1] 钦伦秀[1,2] 刘银坤[1,2] 

机构地区：[1]复旦大学附属中山医院肝癌研究所,上海200032 [2]复旦大学生物医学研究院,上海200032

出　　处：《生物化学与生物物理进展》2009年第10期1275-1282,共8页Progress In Biochemistry and Biophysics

基　　金：supported by a grant from National Basic ResearchProgram of China (001CB510205)~~

摘　　要：在前期研究中发现,氧调节蛋白150(ORP150)是与肝细胞癌相关的糖蛋白.进一步研究了ORP150的表达水平与肝细胞癌的相关性.免疫印迹、细胞免疫化学和定量PCR分别在蛋白质水平和mRNA水平检测了ORP150的表达.运用RNA干扰技术检测了其对凋亡和肝细胞癌侵袭性的影响.发现:无论是蛋白质水平还是mRNA水平,与正常肝细胞相比,ORP150在肝细胞癌中表达明显上调;经RNA干扰后,肝细胞癌的凋亡明显增加,但肿瘤细胞的侵袭性无改变.肝细胞癌中,ORP150表达上调,它可能抑制肿瘤细胞的凋亡而促进其生长.ORP150有可能成为肝细胞癌的治疗靶点.In previous study, the 150-ku oxygen-regulated protein （ORP150） was identified as a candidate gtycoprotein related to hepatocellular carcinoma. In order to further validate the expression level of ORP150 in hepatocellular carcinoma, protein expression was determined by Western blot and cell immunochcmistry, and messenger RNA （mRNA） expression was detected by quantitative real-time polymerase chain reaction. The effect of ORP150 on apoptosis and invasive potential of hepatocellular carcinoma cells was evaluated using the small interference RNA （siRNA） technique. Both the protein and mRNA expression levels of ORP150 were significantly upregulated in hepatocellular carcinoma cell lines compared with a non-tumor human liver cell line. After transfection with the specific siRNA of ORP150, significantly greater apoptosis of hepatocellular carcinoma cells was induced compared with untransfected cells. However, no significant effect on invasive potential was found. Overexpression of ORP150 was associated with hepatocellular carcinoma, and ORP150 might promote the proliferation of hepatocellular carcinoma cells by inhibiting apoptosis. ORP 150 could be a potential therapeutic target for hepatocellular carcinoma.

关 键 词：氧调节蛋白150 人肝细胞癌 凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R733.1[医药卫生—临床医学]