Cytocompatibility of Self-assembled Hydrogel from IKVAV-containing Peptide Amphiphile with Neural Stem Cells  

作　　者：宋玉林 

机构地区：[1]Department of Orthopaedics, the Second Affiliated Hospital of Nanchang University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2009年第5期753-756,共4页武汉理工大学学报（材料科学英文版）

摘　　要：Neural Stem Cells （NSCs） were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile （IKVAV-PA） for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional （3D） hydrogels （Experimental Group, EG） or surface of coverslips （Control Group, CG）, double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 24 h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG （P〈0.05）. The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.Neural Stem Cells （NSCs） were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile （IKVAV-PA） for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional （3D） hydrogels （Experimental Group, EG） or surface of coverslips （Control Group, CG）, double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 24 h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG （P〈0.05）. The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.

关 键 词：IKVAV HYDROGEL CYTOCOMPATIBILITY proliferation 

分 类 号：Q42[生物学—神经生物学]