Biomineralization of the Surface of PLGA-(ASP-PEG) Modified with the K_(16) and RGD-containing Peptide  

作　　者：郝杰 郑启新 

机构地区：[1]Department of Orthopaedic Surgery, First Affiliated Hospital, Chongqing Medical University [2]Department of Orthopaedics , Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2009年第5期768-771,共4页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China (No.30170270, 30200063)

摘　　要：K16 and RGD-containing peptide was used to modify the surface of three-dimensional PLGA-（ASP-PEG） matrix, then the modified PLGA-（ASP-PEG） was incubated in modified simulated body fluid （SBF）. The biomineralization of the modified PLGA- （ASP-PEG） was explored, and the peptide was synthesized with solid phase synthesis technology and linked cova-lently to PLGA-（ASP-PEG） through cross-linker （Sulfo-LC-SPDP）, which was characterized with XPS. The modified PLGA-（ASP-PEG） （Experiment group, EG） and PLGA-（ASP-PEG） （Control group, CG） were all incubated into SBF for 10 d, and the growth of hydroxyapatite （HA） nanocrys-tals was confirmed with XRD, EDS and SEM. HPLC shows that peptide purity is 94.13%, while MS analysis shows that molecular value of peptide is 2741.26. Binding energy of the sulphur in EG was 164 eV is detected by XPS, and the ratio of carbon and sulphur is 99.746：0.1014. SEM analysis demonstrates the better growth of bonelike HA nanocrystals in EG than that in CG. The component of mineral in EG consisted mainly of hydroxyapatite containing low crystalline nanocrystals, and the Ca/P ratio is about 1.60, which is similar to that of natural bone, while the Ca/P ratio in CG is 1.52. PLGA-（ASP-PEG） modified with peptide provided enough functional groups for biomineralization, and possessed the bonelike structure.K16 and RGD-containing peptide was used to modify the surface of three-dimensional PLGA-（ASP-PEG） matrix, then the modified PLGA-（ASP-PEG） was incubated in modified simulated body fluid （SBF）. The biomineralization of the modified PLGA- （ASP-PEG） was explored, and the peptide was synthesized with solid phase synthesis technology and linked cova-lently to PLGA-（ASP-PEG） through cross-linker （Sulfo-LC-SPDP）, which was characterized with XPS. The modified PLGA-（ASP-PEG） （Experiment group, EG） and PLGA-（ASP-PEG） （Control group, CG） were all incubated into SBF for 10 d, and the growth of hydroxyapatite （HA） nanocrys-tals was confirmed with XRD, EDS and SEM. HPLC shows that peptide purity is 94.13%, while MS analysis shows that molecular value of peptide is 2741.26. Binding energy of the sulphur in EG was 164 eV is detected by XPS, and the ratio of carbon and sulphur is 99.746：0.1014. SEM analysis demonstrates the better growth of bonelike HA nanocrystals in EG than that in CG. The component of mineral in EG consisted mainly of hydroxyapatite containing low crystalline nanocrystals, and the Ca/P ratio is about 1.60, which is similar to that of natural bone, while the Ca/P ratio in CG is 1.52. PLGA-（ASP-PEG） modified with peptide provided enough functional groups for biomineralization, and possessed the bonelike structure.

关 键 词：BIOMINERALIZATION PLGA hydroxyapatite （HA） PEPTIDE 

分 类 号：Q50[生物学—生物化学]