胰岛素样生长因子-1对小鼠T,B淋巴细胞分泌功能的影响  

Effect of IGF-I on secretory function of mouse T,B lymphocytes

作　　者：彭菲[1] 左永昌[1] 张玮[1] 

机构地区：[1]井冈山大学医学院免疫学教研室,江西吉安343000

出　　处：《第四军医大学学报》2009年第20期2116-2118,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(39760070)

摘　　要：目的:观察胰岛素样生长因子-1(IGF-1)对T,B细胞分泌功能的影响,以探究IGF-1治疗应激机体免疫功能低下的可能性.方法:用酶联免疫吸附试验(ELISA)分析体外免疫应答中IGF-1对小鼠T细胞分泌白介素-2(IL-2)和B细胞分泌免疫球蛋白(Ig)的影响.结果:小鼠T,B细胞在给予80μg/LIGF-1的条件下培养3d,其上清的IL-2活性比对照组增加了366.74nkat/mL(P<0.01),培养6d后正常小鼠T,B细胞培养上清和应激小鼠T,B细胞培养上清中的IgG含量均比对照组高,尤其是应激小鼠T,B细胞培养上清的IgG含量比对照组增加了41μg/L(P<0.01).结论:抗原刺激后的小鼠T,B细胞进行体外培养时,在IGF-1存在下T细胞分泌IL-2和B细胞分泌IgG的能力增强,提示IGF-1对机体的免疫应答有调节作用.AIM： To explore the possibility of the IGF-1 treatment on immune dysfunction in stressed situation, the insulin-like growth factor-1 （IGF-1） on the effect of T, B cell secretory function was observed. METHODS： Using enzymelinked immunosorbent assay（ELISA） analyzed the effect of IGF-1 on mouse T cell secretion of interleukin-2 （IL-2） and B ceils secretion of immunoglobulin （Ig） in immune response to antigen in vitro. RESULTS： Mice T, B cells administrated 80 g/L IGF-1 were cultured for 3 d, the IL-2 activity in culture supernatant increased 366.74 nkat/mL as compared with the control group （P 〈 0.01 ）, and that were cultured for 6 d the IgG content in normal mice T, B cells culture supernatant and stressed mice T, B cells culture supernatant were higher separately than that control group. Especially the IgG content in stressed mice T, B ceils culture supernatant increased 41 μg/L as compared with the control group（ P 〈 0.01 ）. CONCLUSION： In the presence of IGF-1 the mouse T, B cell stimulated by antigen cultured in vitro, the ability of T cells secreted IL-2 and B cell secreted IgG were enhanced. It is suggest that IGF-1 may have a modulatory effect on the immune response.

关 键 词：胰岛素样生长因子-1 T B淋巴细胞 免疫调节 

分 类 号：R730.3[医药卫生—肿瘤]