放射治疗荷肾癌小鼠肿瘤生长抑制与脾细胞及肿瘤组织IL-2,IFN-γ和IL-10的关系  

Relationship between the inhibition of tumor growth and the expression of the IL-2,IFN-γ and IL-10 in spleen cells and tumor tissue of renal carcinoma in nude mice after radiothrapy

作　　者：王瑶[1] 吕海燕[1] 郭佳[1] 俞伟[2] 马林[2] 汪涛[3] 黄建华[1] 

机构地区：[1]解放军总医院免疫学研究室,北京100853 [2]解放军总医院放射治疗科,北京100853 [3]解放军总医院胸外科,北京100853

出　　处：《第四军医大学学报》2009年第20期2122-2124,共3页Journal of the Fourth Military Medical University

基　　金：北京市自然科学基金(7042060)

摘　　要：目的:观察放射治疗对BALB/c小鼠肾癌移植瘤的抑瘤作用与细胞因子IL-2,IFN-γ和IL-10表达分泌的关系.方法:建立BALB/c小鼠的Renca肾癌细胞移植瘤放射治疗的模型;采用ELISA试剂盒检测小鼠脾淋巴细胞的细胞因子IL-2,IL-10和IFN-γ分泌水平.RT-PCR法检测肿瘤组织IL-2,IL-10和IFN-γ基因的表达水平.结果:治疗组小鼠肾癌移植瘤生长速度较肿瘤对照组明显减慢.放疗组小鼠脾细胞分泌IL-2,IFN-γ及肿瘤组织IL-2,IFN-γ的表达较肿瘤对照组明显升高,IL-10较肿瘤对照组降低.结论:经放射治疗后,小鼠肿瘤生长受到明显抑制,与放疗引发小鼠脾细胞分泌IL-2,IFN-γ和肿瘤组织IL-2,IFN-γ表达分泌增强,IL-10细胞因子降低有关.AIM： To observe inhibiting growth of renal carcinoma after radiation treatment in BALB/c murine and the expression of the IL-2, IFN-γ and IL-10. METHODS： Animal tumor models have been established. The tumor volumes and weight were measured after radiotherapy. The expression of IL-2, IL-10 and IFN-γ protein in splenocytes and tumors were assayed by ELISA and RT-PCR. RESULTS： Tumor regression in radiotherapy more effectively than untreated mice. The expression level of IL-2 and IFN-γ, was remarkably increased, the expression level of IL-10 pretein was significantly decreased after radiation. CONCLUSION ： The inhibition of growth of tumor were associated with the expression level of IL-2 and IFN-γ was remarkably increased.

关 键 词：放射治疗 IL-2 IL-10 IFN-Γ 肾肿瘤 

分 类 号：R737.11[医药卫生—肿瘤]