表达犬细小病毒VP2蛋白的嵌合狂犬病病毒生物学特性的研究  

Biological Characteristics of a Chimeric Rabies Virus Expressing Canine Parvovirus VP2 Protein

作　　者：牛学锋[1] 刘晓慧[1] 孙招金[1] 施赫赫[1] 陈晶[1] 江飙[1] 孙京臣[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院微生物教研室,广州510642

出　　处：《病毒学报》2009年第5期362-367,共6页Chinese Journal of Virology

基　　金：国家"863"项目(2006AA10A204)

摘　　要：为获得狂犬病病毒和犬细小病毒的二联疫苗,本研究运用分子生物学技术在狂犬病病毒基因组的G基因与L基因之间插入犬细小病毒VP2基因,通过反向遗传操作系统拯救获得了含犬细小病毒VP2基因的嵌合狂犬病病毒HEP-Flury(VP2),并研究了该病毒的生物学特性。结果表明,嵌合狂犬病病毒HEP-Flury(VP2)可以在BHK-21细胞上稳定繁殖,在传代10次后,外源基因VP2能高效表达;以HEP-Flury(VP2)免疫小鼠,可以诱导免疫小鼠产生抗狂犬病病毒和抗犬细小病毒的抗体。To obtain a bivalence vaccine against canine rabies virus and canine parvovirus, a chimeric rabies virus expressing canine parvovirus VP2 protein was generated by the technique of reverse genetics. It was shown that the chimeric virus designated as HEP-Flury（VP2） grew well on BHK-21 cells and the VP2 gene could still be stably expressed after ten passages on BHK-21 cells. Experiments on the mice immunized with the chimeric virus HEP-Flury（VP2） demonstrated that specific antibodies against rabies virus and canine parvovirus were induced in immunized mice after vaccination with the live chimeric virus.

关 键 词：狂犬病病毒 反向遗传 犬细小病毒 疫苗 

分 类 号：S852.655[农业科学—基础兽医学]