EPHB6受体型酪氨酸蛋白激酶对ERK信号通路的调控效应  

Regulation of EPHB6 receptor tyrosine kinase on the ERK signaling pathway

作　　者：铁茹[1] 陈宝莹[2] 刘利兵[1] 陈健康[1] 张学策[1] 周幸春[1] 曲萍[1] 黄小军[1] 于军[1] 

机构地区：[1]第四军医大学基础部基础医学教学实验中心,陕西西安710033 [2]第四军医大学唐都医院放射科,陕西西安710038

出　　处：《第四军医大学学报》2009年第19期1864-1867,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30801385)

摘　　要：目的:探讨促红细胞生成素产生肝细胞B6(EPHB6)对于胞外信号调节激酶(ERK)信号转导通路的作用.方法:以非小细胞肺癌细胞系A549作为研究对象,采用Western Blot法检测ERK的磷酸化水平及通路检测(PathDe-tect)法检测ERK下游分子和Ets结构域蛋白1(Elk-1)转录因子的活性.结果:A549细胞中EPHB6的表达导致ERK的磷酸化.相反,siRNA干涉EPHB6的表达可以逆转ERK的磷酸化.并且,EPHB6导致的ERK磷酸化与Elk-1转录因子脱偶联.结论:表明EPHB6对于ERK信号通路具有调控作用.AIM：To investigate the regulatory effects of EPHB6 receptor tyrosine kinase on the signaling pathway of ERK MAPK.METHODS：A549 non-small cell lung cancer cell line was used in the experiments.Western blotting was used to assess the phosphorylation levels of EPHB6,and the status of Elk-1,a transcriptional activator downstream of EPHB6,was determined by PathDetect kit.RESULTS：Expression of EPHB6 induced the expression knocking-down by EPHB6 siRNA of ERK in A549 cells.reversed the phosphorylation of ERK.Moreover,EPHB6-induced phosphorylation of ERK was uncoupled with Elk-1 transcriptional activator.CONCLUSION：EPHB6 plays a role in the regulation of ERK signaling pathway.

关 键 词：EPHB6 受体型酪氨酸蛋白激酶 非小细胞肺癌 细胞外信号调节激酶 有丝分裂原蛋白活化激酶 Elk-1 

分 类 号：R734.2[医药卫生—肿瘤]