四氯化碳和硫代乙酰胺诱导大鼠肝纤维化的对比研究  

Comparison study of hepatic fibrosis in rats induced by carbon tetochloride and tioacetamide

作　　者：张丽娜[1] 龚均[1] 柴宁莉[2] 

机构地区：[1]西安交通大学第二附属医院消化科,陕西西安710004 [2]西安交通大学第三附属医院消化科,陕西西安710061

出　　处：《第四军医大学学报》2009年第19期1909-1912,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30600848)

摘　　要：目的:采用四氯化碳(CCL4)和硫代乙酰胺(thioacetamide,TAA)两种试剂分别制备大鼠肝纤维化模型,探讨两种方法的优缺点及适用范围.方法:将60只SD大鼠随机分为4组,用CCL4和TAA制备肝纤维化模型并分别设立对照组.观察大鼠一般情况并于第28,56日称体质量,测定血清谷丙转氨酶(ALT),谷草转氨酶(AST)以及透明质酸(HA)和IV型胶原(CIV)、肝匀浆超氧化物歧化酶(SOD)和丙二醛(MDA),取肝组织进行HE和Masson染色,分别观察肝组织的损伤程度和胶原增生的情况.结果:第28日,两模型组的各项指标与其对照组比较均有显著差异,而模型组之间无显著差异;第56日,CCL4组各项指标有明显好转,部分指标与对照组无差异,而TAA组的各项指标进一步恶化,部分指标与CCL4组对比有显著差异.结论:CCL4所致的肝纤维化模型更适用于肝纤维化自发逆转机制以及相关治疗策略的研究.TAA所致的肝纤维化模型适用于肝纤维化的机制研究、治疗药物的筛选和肝纤维化血清学标记物的可靠性评价等.AIM：To induce hepatic fibrosis in rats by CCL4 and TAA respectively to compare the advantages and disadvantages of both methods.METHODS：Sixty male Sprague-Dawly rats were randomly divided into four groups.Hepatic fibrosis was induced in the ways introduced in references.Control groups were set respectively.The animals were sacrificed on day 28 and 56 after weighed.Serum was collected to examine the level of ALT,AST,HA and CIV and livers were homogenized to examine the level of SOD and MDA.The liver inflammation and collagen deposition were observed with HE and Masson＇s collagen stains and analyzed with scoring and staging systems.RESULTS：On day 28,significant differences in observed indexes were found between control and model groups,but no difference was seen between the 2 model groups.On day 56,significant differences was observed between the two model groups.CONCLUSION：The animal model of hepatic fibrosis induced by CCL4 is more suitable for researches on the mechanism of spontaneous regression of hepatic fibrosis and related strategies for treatment.The animal model of hepatic fibrosis induced by TAA is more suitable for researches on the mechanism of liver fibrosis,evaluation of antifibrotic effects of medicine,and evaluation of reliability of serous markers for the diagnosis of liver fibrosis.

关 键 词：四氯化碳 硫代乙酰胺 肝纤维化 动物模型 

分 类 号：R575.2[医药卫生—消化系统]