E-钙粘蛋白基因多态性与陕西地区食管癌的关联研究  

Association of CDH1 gene polymorphisms with esophageal squamous cell carcinoma in Shaanxi Province

作　　者：朱少君[1] 张力[1] 张伟[1] 李艳红 朱金燕[1] 巩丽[1] 兰淼[1] 韩秀娟[1] 姚丽[1] 

机构地区：[1]第四军医大学唐都医院病理科,陕西西安710038 [2]第四军医大学唐都医院妇产科,陕西西安710038

出　　处：《第四军医大学学报》2009年第19期1978-1981,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30171052)

摘　　要：目的:探讨CDH1基因启动子区C-160A单核苷酸多态性(SNP)对食管鳞状细胞癌(ESCC)发生、淋巴结转移及预后的影响.方法:采用病例-对照研究,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,分析218例ESCC和218名健康对照者的CDH1C-160ASNP分布情况.结果:ESCC组的CDH1基因启动子区C-160A多态性基因型和等位基因型分布与健康对照组显著性差异(P<0.05).根据吸烟状况分层分析发现,CDH1的基因型及等位基因型频率在吸烟及非吸烟的患者和健康个体中均有显著性差异(P<0.05).根据有无淋巴结转移进行分层分析表明,CDH1基因多态性在有淋巴结转移组和无淋巴结转移组之间无显著性差异(P>0.05),所以CDH1C-160A基因多态性与淋巴结转移的风险性无关.结论:CDH1C-160A多态性基因型和等位基因型与ESCC的发生和发展相关,CDH1C-160A多态性与ESCC患者淋巴结转移和预后无关.AIM：To explore the influence of the single nucleotide polymorphism（SNP）variants in CDH1 on the development and lymphatic metastases of esophageal squamous cell carcinoma（ESCC）.METHODS：We conducted a population-based case control study in 218 patients with esophageal squamous cell carcinoma and 218 healthy controls.CDH1 genotypes were determined by PCR with restriction fragment length polymorphism（PCR-RFLP）.RESULTS：The overall genotype and allelotype distribution of the CDH1 polymorphisms in ESCC patients were significantly different from those in healthy controls（P〈0.05）.Stratification analysis found that in either smoking group or non-smoking group the frequency of CDH1 allele in ESCC and healthy groups is significantly difference（P〈0.05）.The ESCC cases were further stratified according to the status of lymphatic metastasis at the time of diagnosis.No significant difference was observed in the frequencies of the CDH1 genotypes between ESCC patients with positive and negative lymphnode（P〉 0.05）.CONCLUSION：The C-160A of CDH1 polymorphism is associated with the incidence of esophageal squamous cell carcinoma in the Shaanxi Province,but not associated with the lymphatic metastasis and prognosis of ESCC.

关 键 词：食管癌 多态性 单核苷酸多态性 疾病易感性 

分 类 号：R735.1[医药卫生—肿瘤]