PCR-焦磷酸测序技术在沙门菌食物中毒中的应用  

Application of PCR-pyrosequencing technology for food poisoning of Salmonella

作　　者：田会方[1] 徐保红[1] 李秀娟[1] 李波[1] 王晓丽[1] 

机构地区：[1]石家庄市疾病预防控制中心,石家庄050011

出　　处：《中国卫生检验杂志》2009年第11期2584-2585,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:应用PCR-焦磷酸测序技术对食物中毒标本中沙门菌进行快速检测。方法:疑似沙门菌食物中毒的相关标本9份。前增菌后,进行核酸提取及PCR-焦磷酸测序技术检测。同时按GB/T4789.4-2008国标法进行细菌分离培养,利用VITEK全自动微生物鉴定仪对可疑菌进行生化反应检测,血清学试验鉴定血清型。结果:PCR-焦磷酸测序结果显示9份标本中3份沙门菌阳性。用分离鉴定方法也仅在该3份标本中分离出沙门菌。结论:PCR-焦磷酸测序技术可用于沙门菌食物中毒的快速检测。Objective：To apply PCR-Pyrosequencing technology on samples of food poisoning for rapid detection of Salmonella.Methods：After pre-enrichment of 9 specimens,PCR-pyrosequencing assay was used to detect Salmonella.At the same time,isolation and culture of bacteria were performed based on GB/T4789.4-2008.The use of automated microbial identification VITEK instrument carried out the detection of biochemical reactions for suspicious bacteria.Results：3 of 9 samples showed Salmonella positive by PCR-Pyrosequencing.3 strains Salmonella were isolated from 9 samples by traditional methods.Conclusion：PCR-pyrosequencing technology can be used as a rapid method for food poisoning of Salmonella.

关 键 词：沙门菌 PCR 焦磷酸测序 食物中毒 快速检测 

分 类 号：R446.5[医药卫生—诊断学]