PCA3(DD3)基因多态性与前列腺癌风险关系的研究  

Relationship beween polymorphism of PCA3 gene exon and susceptibility to prostate cancer

作　　者：陈占国[1] 陶志华[1] 陈伟[2] 周武[1] 陈蕾[3] 

机构地区：[1]温州医学院附属第一医院实验诊断中心,浙江温州325000 [2]温州医学院附属第一医院泌尿外科,浙江温州325000 [3]温州医学院附属第三医院检验科,浙江瑞安325200

出　　处：《中国卫生检验杂志》2009年第11期2612-2613,2617,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金项目资助(30872421);温州市科技计划项目资助(Y20070122)

摘　　要：目的:探讨PCA3基因多态性与前列腺癌遗传易感性的关系。方法:采用PCR和基因片段直接测序方法,分别检测41例前列腺癌(PCa)患者和40例良性前列腺增生(BPH)患者的PCA3外显子区域的SNP变异情况,分析PCA3基因多态性与PCa的相关性。结果:PCA3基因外显子2区域存在1个SNP位点(A164C),基因型分别为AA型、AC型、CC型;Logistic回归分析显示,AC、CC及AC+CC基因型是PCa的危险因子(OR值=6.171,95%CI=1.877-20.286;OR值=5.400,95%CI 1.421-20.518;OR值=5.891,95%CI=1.915-18.124),等位基因C是PCa的危险因子(OR值=2.353,95%CI=1.252-4.421)。结论:PCA3基因外显子2 SNP A164C多态性与PCa的发病风险高度相关,携带等位基因C的基因型是PCa的遗传危险因素。Objective：To investigate the relationship between polymorphism of PCA3 gene exon and susceptibility to prostate cancer.Methods：Polymerase chain reaction（PCR） and direct sequencing were performed to detect the polymorphism of PCA3 gene exon in 41 patients with prostate cancer（PCa） and 40 patients with prostatic hyperplasia（BPH）.The relationship between polymorphism of PCA3 gene exon and susceptibility to prostate cancer was assessed.Results：We found a SNP（A164C） in PCA3 gene at exon 2 reigon.A/A,A/C,C/C genotypes were identified in PCA3 gene at exon 2 reigon.Logistic regression analysis suggested A/C,C/C,A/C＋C/C genotypes were genetic risk factors for PCa（OR=6.171,95% CI=1.877-20.286;OR=5.400,95% CI=1.421-20.518;OR =5.891,95% CI=1.915-18.124,respectively）.Allete C was genetic risk factors for PCa（OR=2.353,95% CI=1.252-4.421）.Conclusion：The exon 2 polymorphism of PCA3 gene（A164C） is closely associated with human prostate cancer and AC,CC,AC＋CC genotype are genetic risk factors for PCa.

关 键 词：前列腺癌 PCA3 基因 多态性 

分 类 号：R737[医药卫生—肿瘤]