阳离子交换固相萃取在三聚氰胺检测的应用  

Application of solid-phase extraction pretreatment technique on detection of melamine in dairy

作　　者：申书昌[1] 杨建平[1] 李媛媛[1] 商德发[1] 

机构地区：[1]齐齐哈尔大学化工学院分析测试中心,黑龙江齐齐哈尔161006

出　　处：《哈尔滨商业大学学报（自然科学版）》2009年第5期597-599,共3页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省教育厅科学技术研究项目(11531410)

摘　　要：采用阳离子交换固相萃取小柱分离富集液态奶中三聚氰胺，用甲醇洗脱后，以C8反相柱离子对液相色谱分析测试三聚氰胺含量．实验中考察了操作条件对液态奶中三聚氰胺测定结果的影响，对固相萃取前处理条件进行了优化，结果表明，固相萃取前处理过程的最佳洗脱体积8mL，流速0．5mL／min．工作曲线方程为Y=4．0963x＋21．508，相关系数为R^2=0．9978，相对标准偏差小于3．1％，回收率在96．4％-102．9％之间，结果令人满意．In this paper ,solid-phase extraction colmun with cation exchange resin is used for separating and concentrating melamine in dairy. The melamine is eluted with methanol, then it is detected by reversed phase ion pair chromatography with Cs colum. Effects of experiment conditions on the determination resuhs of melamine are inspected. Solid - phase extraction pretreatment conditions are optimized, optimum eluting volume is 8 mL and optimum eluting velocity of flow is 0.5 mL/min. The linearity relation between the concentration of melamine and peak area is y =4. 096 3x ＋ 21. 508, correlation efficient（R2） is 0. 997 8, the relative standard deviation is 3.1% ,sample recovery rate is in the range of 96.4% and 102. 9%. The experiment results are satisfied.

关 键 词：乳品 三聚氰胺 固相萃取 液相色谱 

分 类 号：O657[理学—分析化学]