脑胶质瘤术中荧光显像的实验研究  

Experimental study of intraoperative fluorescence imaging for brain glioma

作　　者：顾小宇[1] 尚寒冰[1] 赵卫国[1] 王晓强[1] 赵开军[1] 强强[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院神经外科,上海200025

出　　处：《中国微侵袭神经外科杂志》2009年第10期456-458,共3页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：国家自然科学基金资助项目(编号:30772222)

摘　　要：目的探讨采用荧光标记白蛋白行胶质瘤术中荧光实时显像的可行性。方法采用化学合成法将荧光素钠标记鼠血清白蛋白。先行体外实验:将C6胶质瘤细胞、星形胶质细胞分别与0.1mg/ml荧光白蛋白培养24h,观察细胞荧光染色情况。再行体内实验:术前24h,在载瘤大鼠静脉内注射荧光白蛋白溶液,术中分别在日光及荧光系统下观察肿瘤与正常脑组织形态,并对荧光处组织取活检行苏木精-伊红染色。结果体外实验:荧光显微镜下,C6细胞发出强绿色荧光,星形胶质细胞荧光较弱。体内实验:术中日光下肿瘤组织呈黄色,荧光系统下呈绿色荧光,正常脑组织无荧光显示;苏木精-伊红染色提示荧光处组织为胶质瘤组织。结论荧光白蛋白引导的胶质瘤术中实时显像能有效帮助术者区分胶质瘤与正常脑组织。Objective To investigate the feasibility of real time fluorescence imaging during glioma surgery by fluorescence-labeled albumin.Methods Rat serum albumin（RSA） was labeled with sodium fluorescein（NaF） by chemical synthesis method.In vitro experiments：C6 glioma cells and astrocytes were incubated with 0.1 mg/ml fluorescent protein for 24 h respectively,and then the expression of fluorescence on the cells was observed.In vivo experiments：the intravenous injection of NaF-RSA solution was given 24 h before rat glioma surgery.The appearances of glioma and normal brain tissues were observed during the surgery under sunlight and fluorescence microscope,and the biopsy of the fluorescent tissues was performed and examined by HE staining.Results C6 glioma cells had bright green fluorescence,while astrocyte cells had slender fluorescence in vitro experiment.The in vivo experiment showed that the glioma tissues were stained yellow under sunlight and green fluorescence under fluorescence microscope,while the normal brain had no fluorescence.HE staining showed that the tissues with green fluorescence were glioma tissues.Conclusion The real time fluorescence imaging technology established by fluorescent albumin could effectively distinguish tumor tissues from normal brain tissues during brain glioma surgery.

关 键 词：神经胶质瘤 荧光素 血清白蛋白 

分 类 号：R739.41[医药卫生—肿瘤] R730.264[医药卫生—临床医学]