粘附分子E-钙粘素/β-连环素在LNCaP和ARCaP亚细胞系中的差异性表达及意义  

Expressions of E-cadherin and β-catenin in LNCaP and ARCaP Cell Lines and Their Significance

作　　者：姜永光[1] 赵佳晖[1] 罗勇[1] 贺大林[2] 李楠[2] 崔新浩[1] 彭涛[1] 

机构地区：[1]首都医科大学附属北京安贞医院泌尿外科,北京100029 [2]西安交通大学医学院第一附属医院泌尿外科,陕西西安710061

出　　处：《中华男科学杂志》2009年第10期867-871,共5页National Journal of Andrology

基　　金：国家自然科学基金(30872934);北京市自然科学基金(5072019)~~

摘　　要：目的:检测粘附分子E-钙粘素/β-连环素(E-cadherin/β-catenin)复合体在LNCaP和ARCaP亚细胞系IF11、IA8中的差异性表达,以探讨E-cadherin/β-catenin复合体与前列腺癌转移侵袭的关系。方法:用Western印迹及免疫荧光鉴定LNCaP和ARCaP亚细胞系IF11、IA8中E-cadherin、β-catenin的表达以及分布情况。结果:Western印迹显示E-cadherin在LNCaP细胞系中表达较高,在IF11、IA8中表达缺失,β-catenin在IF11、IA8中表达显著高于LNCaP(P<0.01)。免疫荧光显示β-catenin在LNCaP细胞系中主要分布在细胞膜上,而在ARCaP亚细胞系IF11、IA8中,主要分布于细胞核中;E-cadherin在LNCaP细胞中主要分布在细胞膜上。结论:不同上皮细胞间质转化态(EMT)特性的前列腺癌细胞系中E-cadherin/β-catenin表达以及分布存在差异,β-catenin的核转位可能是诱发前列腺癌细胞系发生EMT改变的机制之一。Objective： To observe the expressions of E-cadherin and β-catenin in LNCaP and ARCaP cell lines and explore their relationship with the metastasis of human prostate cancer.Methods： The expressions and distribution of E-cadherin and β-catenin in LNCaP and ARCaP cell lines（IF11 and IA8） were detected by Western blot and immunofluorescent staining.Results： The expression of E-cadherin was high in LNCaP,but absent in IF11 and IA8,while β-catenin was expressed highly in IF11 and IA8,but lowly in LNCaP.Immunofluorescent staining showed that E-cadherin was mainly in the membrane of LNCaP,while β-catenin both in the membrane of LNCaP and in the nuclei of IF11 and IA8.Conclusion： E-cadherin and β-catenin are differently expressed and distributed in prostate cancer cell lines with different characteristics of epithelial-mesenchymal transition（EMT）,and the abnormal activation of the β-catenin signal pathway may be involved in the EMT of prostate cancer cells.

关 键 词：Β-连环素 E钙-粘素 前列腺癌 上皮细胞间质转化态 

分 类 号：R737.25[医药卫生—肿瘤]