帕金森病模型大鼠中脑腹侧被盖区酪氨酸羟化酶免疫阳性神经元的改变  

Experimental changes of tyrosine hydroxylase neurons in mesencephalic ventral tegmental area of Parkinson disease rats

作　　者：肖春苟[1] 沈伟哉[2] 郭国庆[2] 周星平[1] 胡日明[1] 邹锦慧 

机构地区：[1]韶关学院医学院人体解剖学教研室,广东韶关512026 [2]暨南大学医学院人体解剖学教研室,广州510630 [3]肇庆高等医学专科学校人体解剖学教研室,肇庆526020

出　　处：《解剖学研究》2009年第5期321-324,329,共5页Anatomy Research

基　　金：863计划项目(2001AA215131)

摘　　要：目的探讨帕金森病(PD)模型大鼠中脑腹侧被盖区(VTA)酪氨酸羟化酶(TH)免疫阳性神经元的改变。方法将6-羟基多巴胺(6-OHDA)分别注入实验组大鼠左侧黑质致密部和中脑被盖腹侧区以建立帕金森病模型,于术后4d、7d、14d、21d、28d腹腔注射阿扑吗啡(apomorphine,APO),观察并记录大鼠行为学变化情况,利用Nissl染色、酪氨酸羟化酶(TH)免疫细胞化学染色观察大鼠中脑腹侧被盖区神经组织及TH免疫阳性神经元的改变。结果APO诱发实验组PD大鼠均向健侧(右侧)旋转,旋转启动时间逐渐缩短,持续时间逐渐延长,旋转速度逐渐加快,至术后2周旋转行为趋于稳定;Nissl染色见实验组PD大鼠损毁侧(左侧)中脑VTA区神经元数目显著减少,尼氏体模糊,颗粒及密度均降低,伴有大量胶质细胞增生,术后2周、4周注射侧神经元数目较术后1周明显减少(P<0.05);实验组PD大鼠损毁侧(左侧)中脑VTA的TH阳性神经元明显减少,神经元胞体轮廓及突起不清晰,TH阳性纤维也明显减少,分布稀疏,术后2周、4周注射侧阳性神经元数目较术后1周明显减少(P<0.05)。结论PD大鼠损毁侧中脑VTA神经组织有明显破坏,TH阳性神经元显著减少,提示中脑腹侧被盖区TH免疫阳性神经元的改变参与了PD模型大鼠的病理学变化。Objective To explore the changes of tyrosine hydroxylase neurons in mesencephalic ventral tegmental area （VTA）of Parkinson disease （PD）rats. Methods Stereotaxic injection of 6-OHDA into the left substantia nigra pars compacta （SNC） and ventral tegmental area （VTA） of rats to develop PD rat model, The behavioral changes induced by apomorphine were observed at 4,7,14,21,28 days after the injection of 6-OHDA ; neuronal alteration of left mesencephalic VTA were studied adopting Nissl＇s staining, and the changes of tyrosine hydroxylase neurons were analyzed by tyrosine hydroxylase （TH） immunohistochemistry and image analysis. Results The PD rats showed abnormal rotary behavior, in the rotation, the start time of PD rats shortened gradually, the continuous time lengthened, and the rotary rate speeded by degrees. The rotary behavior became invariable until 2 weeks after 6-OHDA injection; Serious neuronal alteration in left （the injured side）VTA of PD rats were observed by Nissl＇s staining; The number of tyrosine hydroxylase neurons in left （the injured side）VTA of PD rats, decreased significantly compared to right side throughout the test time. As to the number of tyrosine hydroxylase neurons in left （the injured side）VTA, 2 or 4 weeks after 6-OHDA injection decreased significantly compared to 1 week after 6-OHDA injection in PD group （P〈0.05）. Conclusion The PD rats showed serious neuronal alteration in the injured VTA, tyrosine hydroxylase neurons were lost significantly in the injured VTA of PD rats, Mesencephalic VTA tyrosine hydroxylase neurons take part in the pathologic changes of PD rats.

关 键 词：帕金森病 中脑腹侧被盖区 羟基多巴胺 酪氨酸羟化酶 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]