海洛因依赖大鼠十二指肠胃泌素和生长抑素免疫反应阳性细胞免疫组织化学和形态计量  

Immunohistochemical and morphological study on gastrin and somatostatin immunoreactive cells in duodenum of heroin dependent rat

作　　者：张伟[1] 梁文妹[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵阳550004

出　　处：《解剖学杂志》2009年第5期595-598,共4页Chinese Journal of Anatomy

基　　金：教育部“春晖计划”项目（Z2005-1-52020）;贵州省科技攻关项目（黔科合s字[2007-1048]）

摘　　要：目的：探讨海洛因依赖对大鼠十二指肠胃泌素（Gas）、生长抑素（SS）免疫反应（IR）细胞形态学和功能改变的影响。方法：应用免疫组织化学SABC法。结果：与正常对照组及盐水对照组比较，Gas-IR、SS-IR细胞免疫组织化学显色在海洛因依赖组均增强，阳性细胞数增多；图像分析结果表明，海洛因依赖组Gas—IR、SS-IR细胞的平均灰度值较正常和盐水对照组显著降低，以24d组最低。结论：大鼠十二指肠中的Gas—IR、SS-IR细胞通过细胞数的增加以及增强合成、分泌Gas、SS的方式，参与海洛因依赖期间大鼠机体内的调节过程。Objective： To explore possible function of duodenum gastrin （Gas） and somatostatin （SS） immunoreactive （IR） cells during heroin dependence period. Methods： Immunohistochemical SABC method was applied. Results： As compared with normal control group （NCG） and saline control group （SCG）, immunoreactivity of duodenum Gas-IR and SS-IR ceils in the heroin dependence group （HI）G） increased obviously, and the immunostaining of cells turned more intensive, especially at the 24th day. The results of image analysis showed that the mean grey scale of duodenum Gas-IR and SS-IR cells in HDG decreased obviously as compared with NCG and SCG, especially at the 24th day. Conclusion： The changes of duodenum Gas- IR and SS-IR ceils in HDG suggest that duodenum Gas-IR and SS-IR cells are involved in the regulation of heroin dependence by enhancing secretion of Gas and SS.

关 键 词：胃泌素 生长抑素 十二指肠 海洛因依赖 大鼠 

分 类 号：R361.3[医药卫生—病理学] R595.5[医药卫生—基础医学]