腺病毒介导胞嘧啶脱氨酶基因联合黄连素对直肠癌细胞的杀伤作用  

Adenoviral mediated cytosine deaminase gene combining with berberine in killing rectal cancer cells

作　　者：刘曾旭[1] 余庆[2] 马学强[3] 刘德明[1] 朱雄文[3] 

机构地区：[1]南昌大学基础医学院解剖学教研室,南昌330006 [2]南昌大学图书馆,南昌330031 [3]浙江省台州市立医院,台州318000

出　　处：《解剖学杂志》2009年第5期635-637,646,共4页Chinese Journal of Anatomy

基　　金：浙江省医药卫生科技计划（20068145）

摘　　要：目的：探讨黄连素联合腺病毒介导的胞嘧啶脱氨酶自杀基因（A＆CD）对直肠癌细胞的体外杀伤作用。方法：用重组腺病毒介导外源CD基因转染到人直肠癌细胞株HR-8348，检测病毒的转导效率和CD基因表达。用四甲基偶氮唑盐（MTT法）检测黄连素联合Ad-CD基因对HR-8348细胞存活率及体外旁观者效应的影响。结果：在250μg／ml5-氟胞嘧啶（5-FC）浓度下，0．1、0．3、3．0、30．0μmol／L浓度的黄连素联合5-FC对直肠癌细胞生长抑制率分别为27．70%、42．4％、52．3％、56．3％。3．0μmol／L浓度的黄连素可作为参考用药浓度。在转染和未转染CD基因的HR-8348混合体系中，黄连素联合CD／5-FC对直肠癌细胞具有更强的抑制作用。结论：黄连素可以增强自杀基因系统对直肠癌细胞的杀伤作用，可作为一种增效剂应用于直肠癌的治疗。Objective： To evaluate antitumor effect of berberine and adenovirus-mediated cytosine deaminase （CD） suicide gene on rectal cancer cells in vitro. Methods： Mediated by adenovirus, the CD gene was transfected into HR-8348 rectal cancer cell line. After identification of transfection rate and CD expression, the cell viability and ＂by-stander effect＂ were examined by using MTT assay. Results： Based on 250 μg/ml 5-FC, 0. 1, 0. 3, 3. 0, 30.0 μmol/1 berberine produced growth suppression rates of HR-8348 ceils were 27. 7~, 42.4%, 52. 3% and 56.3%, respectively. The preferable dose of berberine was 3.0μmol/1. Combined of 3.0μmol/1 berberine with 250 μg/ml 5-FC caused more significant suppression of cells mixed with tranfected and non-tranfected CD compared with those treated with 5-FC only. Conclusion： Berberine can enhance the antitumor of CD suicide gene system for rectal cancer cell, suggesting that it has a potential application in the treatment of rectal cancer.

关 键 词：直肠癌细胞 胞嘧啶脱氨酶 黄连素 基因疗法 

分 类 号：R735.7[医药卫生—肿瘤] R734.2[医药卫生—临床医学]