广西毛南族17个Y染色体短串联重复序列基因座遗传多态性  

Genetic polymorphisms of seventeen Y-chromosomeal short tandem repeats loci in Maonan nationality in Guangxi province

作　　者：滕少康[1] 曹林枝[2] 黄世宁[1] 黄昌盛[1] 侯一平[3] 

机构地区：[1]右江民族医学院解剖学教研室,百色533000 [2]右江民族医学院生物化学教研室,百色533000 [3]四川大学华西基础与法医学院,成都610041

出　　处：《解剖学杂志》2009年第5期690-693,共4页Chinese Journal of Anatomy

基　　金：2007年高校青年骨干教师国内访问学者项目;广西教育厅科研项目（桂教科教2006[26]号）

摘　　要：目的：调查17个Y染色体短串联重复序列（Y-STR）基因座及其单倍型在广西毛南族人群中的分布情况。方法：应用AmpF1STR Yfiler^TM荧光标记复合扩增系统，对毛南族208名无关男性个体血样进行17个Y-STR位点的复合扩增，用ABI PRISM310遗传分析仪对扩增产物进行检测分析。结果：DYS456、DYS389I、DYS390、DYS389Ⅱ、DYS458、DYS19、DYS385a＼b、DYS393、DYS391、DYS139、DYS635、DYS392、y-GATA-H4、DYS437、DYS438、DYS448各位点遗传多样性（GD值）分布在0．5852-0．9770之间。17个Y-STR位点共同构成的单倍型205种，其单倍型多样性为0．999785。广西毛南族与其他群体的Y-STR位点等位基因分布差异具有统计学意义。结论：广西毛南族17个Y-STR位点具有丰富的遗传多样性，可为父权鉴定和父系进化研究提供有价值的遗传学资料。Objective： To investigate the Allelic and haplotype frequency distribution of seventeen short tandem repeat loci of Y chromosome in Maonan nationality in Guangxi province. Methods： Seventeen Y-STR loci, of which the template DNAs were extracted from blood samples of 184 unrelated male individuals in Maonan population, were amplified by using the AmpF1STR Yfile^rTM. The PCR products were genotyped with ABI PRISM 310 genetic analyzer. Results： The gene diversity ranged from 0. 585 2 to 0. 977 0 at DYS456, DYS389 Ⅰ , DYS390, DYS389 Ⅱ, DYS458, DYS19, DYS385a／ b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYSI38 and DYS448. A total of 205 different haplotypes were observed. The haplotype diversity value calculated from all 17 loci combined was 0. 999 785. The significant difference of the allelic frequency distribution in Y-STR loci was observed between Maonan population and other observed populations. Conclusion： The 17 Y-STR loci in Maonan population of Guangxi province are highly affluent genetic polymorphic and can offer valuable genetic data for paternity testing and paternal genetic lineages evolution.

关 键 词：Y染色体 短串联重复序列 毛南族 遗传多态性 

分 类 号：R394[医药卫生—医学遗传学] R698.2[医药卫生—基础医学]